PRRX1 Antibody (OTI1E10)

Novus Biologicals | Catalog # NBP2-45407

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E10
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Product Specifications

Immunogen

Full length human recombinant protein of human PRRX1 (NP_073207) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PRRX1 Antibody (OTI1E10)

Western Blot: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Western Blot: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Western Blot: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRRX1.
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (OTI1E10) [NBP2-45407]

Immunohistochemistry: PRRX1 Antibody (1E10) [NBP2-45407] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for PRRX1 Antibody (OTI1E10)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PRRX1

The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription co-activator, enhancing the DNA-binding activity of serum response factor, a protein required for the induction of genes by growth and differentiation factors. The protein regulates muscle creatine kinase, indicating a role in the establishment of diverse mesodermal muscle types. Alternative splicing yields two isoforms that differ in abundance and expression patterns.

Alternate Names

Homeobox protein PHOX1, paired mesoderm homeo box 1, paired mesoderm homeobox 1 isoform pmx-1b, paired mesoderm homeobox protein 1, paired related homeobox 1, Paired-related homeobox protein 1, PHOX1PRX-1, PMX1PRX1

Gene Symbol

PRRX1

Additional PRRX1 Products

Product Documents for PRRX1 Antibody (OTI1E10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PRRX1 Antibody (OTI1E10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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