Recombinant Human PSD-95 GST (N-Term) Protein

Novus Biologicals | Catalog # H00001742-P01

Novus Biologicals
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Key Product Details

Source

Wheat germ

Tag

GST (N-Term)

Applications

Western Blot, ELISA, Affinity Purification, Functional Assay, Microarray
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Product Specifications

Description

A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-767 of Human DLG4

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MSQRPRAPRSALWLLAPPLLRWAPPLLTVLHSDLFQALLDILDYYEASLSESQKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKVMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFGDVLHVIDASDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

111.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.

Application Notes

In vitro assay was reported in scientific literature (PMID 23260144). Use in ELISA reported in scientific literature (PMID 23371365).

Protein / Peptide Type

Recombinant Protein

Reviewed Applications

Read 1 review rated 4 using H00001742-P01 in the following applications:

Scientific Data Images for Recombinant Human PSD-95 GST (N-Term) Protein

SDS-PAGE: Recombinant Human PSD-95 GST (N-Term) Protein [H00001742-P01]

SDS-PAGE: Recombinant Human PSD-95 GST (N-Term) Protein [H00001742-P01]

SDS-Page: Recombinant Human PSD-95 Protein [H00001742-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Formulation, Preparation, and Storage

H00001742-P01
Preparation Method in vitro wheat germ expression system
Formulation 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -80C. Avoid freeze-thaw cycles.

Background: PSD-95

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Postsynaptic Density Protein 95/Disks Large Homolog 4

Alternate Names

DLG4, PSD95, SAP90

Gene Symbol

DLG4

Additional PSD-95 Products

Product Documents for Recombinant Human PSD-95 GST (N-Term) Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human PSD-95 GST (N-Term) Protein

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant Human PSD-95 GST (N-Term) Protein

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  • Recombinant Human PSD-95 GST (N-Term) Protein
    Name: Tanusree Sen
    Application: In vitro kinase assay
    Verified Customer | Posted 09/10/2019
    Effect of GSK2656157 on CREB phosphorylation and dendritic outgrowth.
    Recombinant Human PSD-95 GST (N-Term) Protein H00001742-P01

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