PSMA1 Antibody (5X3A6)

Western Blot: PSMA1 Antibody (5X3A6) [NBP3-16190]

Western Blot: PSMA1 Antibody (5X3A6) [NBP3-16190] - Western blot analysis of extracts of various cell lines, using PSMA1 Rabbit mAb (NBP3-16190) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Immunocytochemistry/ Immunofluorescence: PSMA1 Antibody (5X3A6) [NBP3-16190] -

Immunocytochemistry/ Immunofluorescence: PSMA1 Antibody (5X3A6) [NBP3-16190] - Confocal imaging of C2C12 cells using PSMA1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Immunocytochemistry/ Immunofluorescence: PSMA1 Antibody (5X3A6) [NBP3-16190] -

Immunocytochemistry/ Immunofluorescence: PSMA1 Antibody (5X3A6) [NBP3-16190] - Confocal imaging of NIH/3T3 cells using PSMA1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.