PSMC1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-14857
![Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP2-14857]](https://resources.rndsystems.com/images/products/PSMC1-Antibody-Immunohistochemistry-NBP2-14857-img0010.jpg "Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP2-14857]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody maps to a region between residue 390 and 440 of human Proteasome (prosome, macropain) Subunit, ATPase, 1 using the numbering given in entry NP_002793.2 (GeneID 5700).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PSMC1 Antibody - BSA Free
Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP2-14857]
Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP2-14857] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- PSMC1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP2-14857]](https://resources.rndsystems.com/images/products/PSMC1-Antibody-Immunohistochemistry-NBP2-14857-img0009.jpg "Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP2-14857]")
Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP2-14857]
Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP2-14857] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PSMC1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Immunoprecipitation: PSMC1 Antibody [NBP2-14857]](https://resources.rndsystems.com/images/products/PSMC1-Antibody-Immunoprecipitation-NBP2-14857-img0001.jpg "Immunoprecipitation: PSMC1 Antibody [NBP2-14857]")
Immunoprecipitation: PSMC1 Antibody [NBP2-14857]
Immunoprecipitation: PSMC1 Antibody [NBP2-14857] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMC1 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. PSMC1 was successfully immunoprecipitated by rabbit anti-PSMC1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).Applications for PSMC1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PSMC1
Alternate Names
26S protease regulatory subunit 4, 26S proteasome AAA-ATPase subunit RPT2, MGC24583, P26S4, p56, proteasome (prosome, macropain) 26S subunit, ATPase, 1, proteasome 26S ATPase subunit 1, Proteasome 26S subunit ATPase 1, proteasome 26S subunit, ATPase, 1, S4MGC8541
Gene Symbol
PSMC1
UniProt
Additional PSMC1 Products
Product Documents for PSMC1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PSMC1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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