PTH Antibody (MSVA-525R)

Novus Biologicals | Catalog # NBP3-57225

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-525R
View all PTH Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide around aa 1-34 of human mature-PTH-polypeptide (exact sequence is proprietary) Swissprot P01270

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Pituitary gland: A strong PTH immunostaining should be seen in all epithelial cells..

Negative Control
Negitve control:Kidney: PTH immunostaining should be absent in all cells.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PTH Antibody (MSVA-525R)

PTH Antibody (MSVA-525R)

Immunohistochemistry-Paraffin:PTH Antibody (MSVA-525R)NBP3-57225

Parathyroid Strong cytoplasmic PTH immunostaining of all cells of a parathyroid gland.
PTH Antibody (MSVA-525R)

Immunohistochemistry-Paraffin:PTH Antibody (MSVA-525R)NBP3-57225

Parathyroid Parathyroid gland showing intense cytoplasmic PTH immunostaining of all glandular cells.
PTH Antibody (MSVA-525R)

Immunohistochemistry-Paraffin:PTH Antibody (MSVA-525R)NBP3-57225

Kidney cortex 10

Applications for PTH Antibody (MSVA-525R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PTH

Parathyroid hormone (PTH), which is also designated parathyrin, is an 84 amino acid single chain peptide that functions to regulate calcium metabolism by raising blood levels of calcium through various mechanisms. PTH stimulates bone formation to increase bone mass and strength in rats and humans. Within the PTH molecule, the essential activity is associated with the first 34 amino acids at the amino-terminus of the molecule. The gene encoding PTH maps to human chromosome 11p15.3-p15.1. Parathyroid hormone-related protein (PTH-rP) is an autocrine factor that is structurally related to PTH yet, unlike PTH, which is synthesized only by the parathyroid cells, PTH-rP is synthesized by several cell types. PTH-rP regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. Isolated from the culture medium of a human lung cancer cell line, PTH-rP produces PTH-like effects that are characterized as humoral hypercalcemia of malignancy.

Long Name

Parathyroid Hormone

Alternate Names

Parathormone, Parathyrin

Gene Symbol

PTH

Additional PTH Products

Product Documents for PTH Antibody (MSVA-525R)

Certificate of Analysis

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Product Specific Notices for PTH Antibody (MSVA-525R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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