PTPRB Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-88829

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
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Product Specifications

Immunogen

Peptide with sequence C-ENHSFSQERTVPDK corresponding to internal region according to NP_001103224.1, NP_002828.3.

Epitope

ENHSFSQERTVPDK - internal region

Reactivity Notes

Expected species reactivity based on sequence similarity: Rat, Porcine, Canine, Bovine.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PTPRB Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PTPRB Antibody [NBP2-88829]

Immunocytochemistry/ Immunofluorescence: PTPRB Antibody [NBP2-88829]

Immunocytochemistry/Immunofluorescence: PTPRB Antibody [NBP2-88829] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: PTPRB Antibody [NBP2-88829]

Flow Cytometry: PTPRB Antibody [NBP2-88829]

Flow Cytometry: PTPRB Antibody [NBP2-88829] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for PTPRB Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:16000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.5 mg/ml in Tris saline pH7.3, 0.5% bovine serum albumin

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PTPRB

PTPRB is a member of the protein tyrosine phosphatase (PTP) family. The family comprises at least 37 proteins, characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. PTP1B is a transmembrane PTP. The PTPs have high substrate specificity for phosphotyrosyl proteins, at the primary sequence level sharing little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. PTP beta interacts with and regulates the tyrosine phosphorylation level of catenins, which are critical in physiological and pathological events such as cell migration, adhesion and transformation.

Alternate Names

DKFZp686E2262, DKFZp686H15164, EC 3.1.3.48, FLJ44133, HPTPB, HPTP-BETA, MGC142023, MGC59935, protein tyrosine phosphatase, receptor type, B, protein tyrosine phosphatase, receptor type, beta polypeptide, Protein-tyrosine phosphatase beta, PTPB, receptor-type tyrosine-protein phosphatase beta, R-PTP-BETA, Vascular endothelial protein tyrosine phosphatase, VEPTP, VE-PTP

Gene Symbol

PTPRB

Additional PTPRB Products

Product Documents for PTPRB Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PTPRB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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