Puromycin-sensitive aminopeptidase/NPEPPS Antibody

Novus Biologicals | Catalog # NBP3-48506

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Puromycin-sensitive aminopeptidase/NPEPPS Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Puromycin-sensitive aminopeptidase/NPEPPS. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Puromycin-sensitive aminopeptidase/NPEPPS Antibody

Puromycin-sensitive aminopeptidase/NPEPPS Antibody

Western Blot: Puromycin-sensitive aminopeptidase/NPEPPS Antibody [NBP3-48506] -

Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Puromycin-sensitive aminopeptidase/NPEPPS antibody [N3C2-2], Internal (NBP3-48506) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Puromycin-sensitive aminopeptidase/NPEPPS Antibody

Immunocytochemistry/ Immunofluorescence: Puromycin-sensitive aminopeptidase/NPEPPS Antibody [NBP3-48506] -

Puromycin-sensitive aminopeptidase/NPEPPS antibody [N3C2-2], Internal detects Puromycin-sensitive aminopeptidase/NPEPPS protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Puromycin-sensitive aminopeptidase/NPEPPS stained by Puromycin-sensitive aminopeptidase/NPEPPS antibody [N3C2-2], Internal (NBP3-48506) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Scale bar= 10 um.
Puromycin-sensitive aminopeptidase/NPEPPS Antibody

Western Blot: Puromycin-sensitive aminopeptidase/NPEPPS Antibody [NBP3-48506] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Puromycin-sensitive aminopeptidase/NPEPPS antibody [N3C2-2], Internal (NBP3-48506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Puromycin-sensitive aminopeptidase/NPEPPS Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Puromycin-sensitive aminopeptidase/NPEPPS

NPEPPS encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq]

Alternate Names

AAP-S, MP100, NPEPPS

Gene Symbol

NPEPPS

Additional Puromycin-sensitive aminopeptidase/NPEPPS Products

Product Documents for Puromycin-sensitive aminopeptidase/NPEPPS Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Puromycin-sensitive aminopeptidase/NPEPPS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Metalloproteases and Regulators

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Protocols

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