PYCR1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # H00005831-B01P

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Format

Azide and BSA Free
View all PYCR1 Primary Antibodies »

Product Specifications

Immunogen

PYCR1 (NP_008838.2, 1 a.a. - 319 a.a.) full-length human protein. MSVGFIGAGQLAFALAKGFTAAGVLAAHKIMASSPDMDLATVSALRKMGVKLTPHNKETVQHSDVLFLAVKPHIIPFILDEIGADIEDRHIVVSCAAGVTISSIEKKLSAFRPAPRVIRCMTNTPVVVREGATVYATGTHAQVEDGRLMEQLLSSVGFCTEVEEDLIDAVTGLSGSGPAYAFTALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLHSEQHPGQLKDNVSSPGGATIHALHVLESGGFRSLLINAVEASCIRTRELQSMADQEQVSPAAIKKTILDKVKLDSPAGTALSPSGHTKLLPRSLAPAGKD

Specificity

PYCR1 - pyrroline-5-carboxylate reductase 1,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for PYCR1 Antibody - Azide and BSA Free

Western Blot: PYCR1 Antibody [H00005831-B01P]

Western Blot: PYCR1 Antibody [H00005831-B01P]

Western Blot: PYCR1 Antibody [H00005831-B01P] - Pig liver extracts. Lane 1: 30 ug, Lane 2: 45 ug, Lane 3: 60 ug, Lane 4: secondary anti-mouse antibody only. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: PYCR1 Antibody [H00005831-B01P]

Immunocytochemistry/ Immunofluorescence: PYCR1 Antibody [H00005831-B01P]

Immunocytochemistry/Immunofluorescence: PYCR1 Antibody [H00005831-B01P] - Analysis of purified antibody to PYCR1 on HeLa cell (antibody concentration 10 ug/mL).
Western Blot: PYCR1 Antibody [H00005831-B01P]

Western Blot: PYCR1 Antibody [H00005831-B01P]

Western Blot: PYCR1 Antibody [H00005831-B01P] - Expression in U-2 OS.
Western Blot: PYCR1 Antibody [H00005831-B01P]

Western Blot: PYCR1 Antibody [H00005831-B01P]

Western Blot: PYCR1 Antibody [H00005831-B01P] - Analysis of PYCR1 expression in transfected 293T cell line by PYCR1 polyclonal antibody. Lane 1: PYCR1 transfected lysate (35.09 kDa). Lane 2: Non-transfected lysate.

Applications for PYCR1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IF. Use in porcine reported by customer review.

Reviewed Applications

Read 1 review rated 4 using H00005831-B01P in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PYCR1

This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternate splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Alternate Names

ARCL2B, EC 1.5.1.2, mitochondrial pyrroline-5-carboxylate reductase 1, P5C, P5C reductase 1, P5CR, P5CR 1, PIG45, PP222, PRO3, proliferation-inducing protein 45, PYCR, pyrroline-5-carboxylate reductase 1, pyrroline-5-carboxylate reductase 1, mitochondrial

Entrez Gene IDs

5831 (Human)

Gene Symbol

PYCR1

UniProt

NP_008838.2 (Human)

Additional PYCR1 Products

Product Documents for PYCR1 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PYCR1 Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PYCR1 Antibody - Azide and BSA Free

Customer Reviews for PYCR1 Antibody - Azide and BSA Free (1)

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Customer Images

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  • PYCR1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Guinea Pig Liver Extract and Pig liver extract
    Species: Pig tissue
    Verified Customer | Posted 04/22/2019
    Lane 1: 30ug pig liver Lane 2 : 45ug pig liver Lane 3 : 60ug pig liver Lane 4 : only 2nd antibody
    * Primary Antibody dilution- 1:500 (overnight in 4°C) * Secondary Antibody dilution - 1:7,500 (anti-mouse) * 5% Blocking Solution * 125 mM NaCl in TBS-t * Exposure Time – 2min10sec with Pento ECL * Expected Molecular Weight – 35.09 KDa
    PYCR1 Antibody - Azide and BSA Free H00005831-B01P

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