PYCR2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-93882

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all PYCR2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 271-320 of human PYCR2 (NP_037460.2). MADQEKISPAALKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKKD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PYCR2 Antibody - Azide and BSA Free

PYCR2 Antibody - Azide and BSA Free

Western Blot: PYCR2 Antibody - Azide and BSA Free [PYCR2] -

Western Blot: PYCR2 Antibody - Azide and BSA Free [PYCR2] - Western blot analysis of various lysates, using PYCR2 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunocytochemistry/ Immunofluorescence: PYCR2 Antibody - Azide and BSA Free [NBP2-93882]

Immunocytochemistry/ Immunofluorescence: PYCR2 Antibody - Azide and BSA Free [NBP2-93882]

Immunocytochemistry/Immunofluorescence: PYCR2 Antibody [NBP2-93882] - Analysis of C6 cells using PYCR2 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: PYCR2 Antibody - Azide and BSA Free [NBP2-93882]

Immunohistochemistry-Paraffin: PYCR2 Antibody - Azide and BSA Free [NBP2-93882]

Immunohistochemistry-Paraffin: PYCR2 Antibody [NBP2-93882] - Human liver cancer using PYCR2 Rabbit pAb at dilution of 1:100 (40x lens).
Immunocytochemistry/ Immunofluorescence: PYCR2 Antibody - Azide and BSA Free [NBP2-93882]

Immunocytochemistry/ Immunofluorescence: PYCR2 Antibody - Azide and BSA Free [NBP2-93882]

Immunocytochemistry/Immunofluorescence: PYCR2 Antibody [NBP2-93882] - Analysis of HeLa cells using PYCR2 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: PYCR2 Antibody - Azide and BSA Free [NBP2-93882]

Immunocytochemistry/ Immunofluorescence: PYCR2 Antibody - Azide and BSA Free [NBP2-93882]

Immunocytochemistry/Immunofluorescence: PYCR2 Antibody [NBP2-93882] - Analysis of L929 cells using PYCR2 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: PYCR2 Antibody - Azide and BSA Free [NBP2-93882]

Immunohistochemistry-Paraffin: PYCR2 Antibody - Azide and BSA Free [NBP2-93882]

Immunohistochemistry-Paraffin: PYCR2 Antibody [NBP2-93882] - Mouse kidney using PYCR2 Rabbit pAb at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: PYCR2 Antibody - Azide and BSA Free [NBP2-93882]

Immunohistochemistry-Paraffin: PYCR2 Antibody - Azide and BSA Free [NBP2-93882]

Immunohistochemistry-Paraffin: PYCR2 Antibody [NBP2-93882] - Rat liver using PYCR2 Rabbit pAb at dilution of 1:100 (40x lens).

Applications for PYCR2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PYCR2

Alternate Names

EC 1.5.1.2, FLJ54750, P5C reductase 2, P5CR 2, P5CR2, pyrroline 5-carboxylate reductase isoform, pyrroline-5-carboxylate reductase 2, pyrroline-5-carboxylate reductase family, member 2

Gene Symbol

PYCR2

Additional PYCR2 Products

Product Documents for PYCR2 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PYCR2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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