Pyruvate Carboxylase Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-93781

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Pyruvate Carboxylase/PC Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 879-1178 of human Pyruvate Carboxylase (NP_001035806.1). HSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pyruvate Carboxylase Antibody - BSA Free

Pyruvate Carboxylase Antibody - BSA Free

Western Blot: Pyruvate Carboxylase Antibody - BSA Free [NBP2-93781] -

Western Blot: Pyruvate Carboxylase Antibody - BSA Free [NBP2-93781] - Western blot analysis of various lysates, using Pyruvate Carboxylase Rabbit pAb (A6301) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunoprecipitation: Pyruvate Carboxylase Antibody - BSA Free [NBP2-93781]

Immunoprecipitation: Pyruvate Carboxylase Antibody - BSA Free [NBP2-93781]

Immunoprecipitation: Pyruvate Carboxylase Antibody [NBP2-93781] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Pyruvate Carboxylase antibody (NBP2-93781). Western blot was performed from the immunoprecipitate using Pyruvate Carboxylase antibody (NBP2-93781) at a dilution of 1:1000.
Immunocytochemistry/ Immunofluorescence: Pyruvate Carboxylase Antibody - BSA Free [NBP2-93781]

Immunocytochemistry/ Immunofluorescence: Pyruvate Carboxylase Antibody - BSA Free [NBP2-93781]

Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP2-93781] - Analysis of U2OS cells using Pyruvate Carboxylase. Blue: DAPI for nuclear staining.

Applications for Pyruvate Carboxylase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:100

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Pyruvate Carboxylase/PC

PCB encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq]

Long Name

Pyruvate Carboxylase

Alternate Names

PC, PCB

Gene Symbol

PC

Additional Pyruvate Carboxylase/PC Products

Product Documents for Pyruvate Carboxylase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Pyruvate Carboxylase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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