Quantikine Immunoassay Control Set 778 Human Serpin E1/PAI-1

R&D Systems | Catalog # QC157

For use with catalog number DSE100
R&D Systems
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Key Product Details

Species

Human

Applications

Control, ELISA

Product Summary for Quantikine Immunoassay Control Set 778 Human Serpin E1/PAI-1

INTENDED USE & DESCRIPTION

For use as quantitative controls for the determination of cytokine concentrations in biological fluids. Concentrations have been assigned using R&D Systems’ Quantikine® kits. Controls are prepared in a buffered protein solution with preservatives. They contain recombinant human cytokines at low, medium and high concentrations. Controls are supplied lyophilized.

STORAGE & STABILITY

Unreconstituted Controls should be stored at 2-8 °C and are stable for at least 6 months from date of receipt. Depending on the analyte of interest, reconstituted controls may be stable when stored at < -20 °C. Users should evaluate the frozen stability of the controls in their application or discard after use.

REAGENT PREPARATION

Reconstitute each vial with the volume of deionized or distilled water indicated on the product datasheet.

PROCEDURE & EXPECTED VALUES

Controls should be assayed in the same manner as unknown specimens.

The acceptable ranges for the analytes in these controls are printed on the product datasheet. Due to possible variations in techniques and methodologies, it is recommended that each laboratory determine its own target range. Laboratories using other test systems should establish their own acceptable ranges as these assays may produce different values.

TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE 

• The ranges were determined using R&D Systems’ Quantikine kits. If expected values are not obtained, verify that the lot numbers on the vials correspond with the lot numbers listed above and the correct volume of deionized or distilled water was used for reconstitution of the controls. 

• The results obtained with these controls depend upon several factors associated with methods and instrumentation. Test systems other than those supplied by R&D Systems may result in values that differ from those printed on this product datasheet.

Formulation, Preparation, and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Serpin E1/PAI-1

Serpin E1, also known as Plasminogen Activator Inhibitor-1 (PAI-1), is the primary inhibitor of urokinase-type and tissue-type plasminogen activators (uPA and tPA) which convert Plasminogen to Plasmin. The PA-Plasmin system is involved in fibrinolysis, tissue fibrosis, angiogenesis, wound healing, tumor cell invasion and metastasis, and obesity. Serpin E1 can promote clot formation, arterial re-endothelialization, and neointima formation while protecting against vascular intima thickening. It binds Vitronectin and inhibits vascular smooth muscle cell and endothelial cell adhesion, proliferation, and motility. Serpin E1 binds and induces the LRP-dependent internalization of cell surface complexes containing uPA, uPAR, and Integrin alpha V beta 3. Serpin E1 secretion is upregulated during inflammation, physical injury, and exposure to Angiotensin II.

Long Name

Plasminogen Activator Inhibitor

Alternate Names

Nexin, PAI-1, PAI1, PLANH1

Entrez Gene IDs

5054 (Human); 18787 (Mouse)

Gene Symbol

SERPINE1

Additional Serpin E1/PAI-1 Products

Product Documents for Quantikine Immunoassay Control Set 778 Human Serpin E1/PAI-1

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Quantikine Immunoassay Control Set 778 Human Serpin E1/PAI-1

For research use only

Customer Reviews for Quantikine Immunoassay Control Set 778 Human Serpin E1/PAI-1 (3)

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  • Quantikine Immunoassay Control Set 778 Human Serpin E1/PAI-1
    Name: Anonymous
    Verified Customer | Posted 12/24/2020
    RnD Low in wells 3B and 4B RnD High in wells 11H and 12H
    Quantikine Immunoassay Control Set 778 Human Serpin E1/PAI-1 QC157
  • Quantikine Immunoassay Control Set 778 Human Serpin E1/PAI-1
    Name: Anonymous
    Verified Customer | Posted 12/24/2020
    QC low found in wells B3&amp;B4 and QC high found in wells H11&amp;H12. Signal was robust and within expected ranges!
    Quantikine Immunoassay Control Set 778 Human Serpin E1/PAI-1 QC157
  • Quantikine Immunoassay Control Set 778 Human Serpin E1/PAI-1
    Name: Anonymous
    Verified Customer | Posted 12/16/2020
    Great QC with results within the given range. The medium QC was replaced with an internal QC. Low is found in wells B3 and B4 (duplicate), and High is located in wells H11 and H12(duplicate). %CV was very low.
    Quantikine Immunoassay Control Set 778 Human Serpin E1/PAI-1 QC157

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Associated Pathways

Blood Coagulation Signaling Pathways Blood Coagulation Signaling Pathway Thumbnail