Serpin E1, also known as Plasminogen Activator Inhibitor-1 (PAI-1), is the primary inhibitor of urokinase-type and tissue-type plasminogen activators (uPA and tPA) which convert Plasminogen to Plasmin. The PA-Plasmin system is involved in fibrinolysis, tissue fibrosis, angiogenesis, wound healing, tumor cell invasion and metastasis, and obesity. Serpin E1 can promote clot formation, arterial re-endothelialization, and neointima formation while protecting against vascular intima thickening. It binds Vitronectin and inhibits vascular smooth muscle cell and endothelial cell adhesion, proliferation, and motility. Serpin E1 binds and induces the LRP-dependent internalization of cell surface complexes containing uPA, uPAR, and Integrin alpha V beta 3. Serpin E1 secretion is upregulated during inflammation, physical injury, and exposure to Angiotensin II.
Human Serpin E1/PAI-1 Quantikine ELISA Kit
R&D Systems | Catalog # DSE100
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Citrate Plasma (50 µL), CTAD Plasma (50 µL)
Sensitivity
0.142 ng/mL
Assay Range
0.312-20 ng/mL (Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, CTAD Plasma)
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Product Summary for Human Serpin E1/PAI-1 Quantikine ELISA Kit
The Quantikine Human Serpin E1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Serpin E1 in cell culture supernates, cell lysates, and plasma. It contains Sf 21-expressed recombinant human Serpin E1 and has been shown to accurately quantitate the recombinant factor in its active and latent forms as well as in the vitronectin complexes. However, the kit does not detect recombinant human Serpin E1 in complexes with recombinant human uPA and recombinant human tPA. Results obtained using natural human Serpin E1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Serpin E1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human Serpin E1
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, Citrate Plasma, CTAD Plasma, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 2.6 | 6.5 | 12.6 | 2.3 | 6.4 | 13.1 |
| Standard Deviation | 0.12 | 0.52 | 0.98 | 0.20 | 0.40 | 0.80 |
| CV% | 4.4 | 8.0 | 7.8 | 9.5 | 6.4 | 6.1 |
Recovery for Human Serpin E1/PAI-1 Quantikine ELISA Kit
The recovery of Serpin E1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 106 | 89-113 |
| Citrate Plasma (n=4) | 99 | 88-112 |
| CTAD Plasma (n=4) | 100 | 83-112 |
| EDTA Plasma (n=4) | 100 | 85-115 |
| Heparin Plasma (n=4) | 98 | 85-113 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Serpin E1 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Serpin E1/PAI-1 Quantikine ELISA Kit
Human Serpin E1/PAI-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Serpin E1/PAI-1
Long Name
Plasminogen Activator Inhibitor
Alternate Names
Nexin, PAI-1, PAI1, PLANH1
Gene Symbol
SERPINE1
Additional Serpin E1/PAI-1 Products
Product Documents for Human Serpin E1/PAI-1 Quantikine ELISA Kit
Product Specific Notices for Human Serpin E1/PAI-1 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Human Serpin E1/PAI-1 Quantikine ELISA Kit
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Customer Reviews for Human Serpin E1/PAI-1 Quantikine ELISA Kit (5)
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Sample Tested: Plasma and UrineVerified Customer | Posted 11/03/2023Measured plasma & urine at 2-fold dilution
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Sample Tested: PlasmaVerified Customer | Posted 12/24/2020Standard curve in duplicate in Columns 1 and 2
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Sample Tested: EDTA PlasmaVerified Customer | Posted 12/24/2020Comprehensive and accurate assay that was easy to execute
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Sample Tested: PlasmaVerified Customer | Posted 12/16/2020Great assay with a very clear protocol. Produced results as expected.
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Sample Tested: cell culture supernatantsVerified Customer | Posted 07/20/2017used at 1:2 dilution for cell culture supernatants
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Protocols
View specific protocols for Human Serpin E1/PAI-1 Quantikine ELISA Kit (DSE100):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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