Human Serpin E1/PAI-1 Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, CTAD Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean | 2.6 | 6.5 | 12.6 | 2.3 | 6.4 | 13.1 |
Standard Deviation | 0.12 | 0.52 | 0.98 | 0.2 | 0.4 | 0.8 |
CV% | 4.4 | 8 | 7.8 | 9.5 | 6.4 | 6.1 |
Recovery
The recovery of Serpin E1 spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 106 | 89-113 |
Citrate Plasma (n=4) | 99 | 88-112 |
CTAD Plasma (n=4) | 100 | 83-112 |
EDTA Plasma (n=4) | 100 | 85-115 |
Heparin Plasma (n=4) | 98 | 85-113 |
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background: Serpin E1/PAI-1
The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.





Citations for Human Serpin E1/PAI-1 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Anti ?-enolase antibody is a novel autoimmune biomarker for unexplained recurrent miscarriages
Authors: Y Ye, C Kuhn, M Kösters, GJ Arnold, H Ishikawa-A, C Schulz, N Rogenhofer, CJ Thaler, S Mahner, T Fröhlich, U Jeschke, V von Schönf
EBioMedicine, 2019;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Senolytics in idiopathic pulmonary fibrosis: Results from a first-in-human, open-label, pilot study
Authors: JN Justice, AM Nambiar, T Tchkonia, NK LeBrasseur, R Pascual, SK Hashmi, L Prata, MM Masternak, SB Kritchevsk, N Musi, JL Kirkland
EBioMedicine, 2019;0(0):.
Species: Human
Sample Types: Serum
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Prostaglandin E2 receptor 3 (EP3) signaling is induced in placentas with unexplained recurrent pregnancy losses (uRPL)
Authors: Y Ye, A Vattai, N Ditsch, C Kuhn, M Rahmeh, S Mahner, M Ripphahn, R Immler, M Sperandio, U Jeschke, V von Schoen
Endocr Connect, 2018;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Combined serum levels of multiple proteins in tPA-BDNF pathway may aid the diagnosis of five mental disorders
Authors: S Chen, H Jiang, Y Liu, Z Hou, Y Yue, Y Zhang, F Zhao, Z Xu, Y Li, X Mou, L Li, T Wang, J Zhao, C Han, Y Sui, M Wang, Z Yang, Y Lu, Y Zhu, J Li, X Shen, F Sun, Q Chen, H Chen, Y Yuan
Sci Rep, 2017;7(1):6871.
Species: Human
Sample Types: Serum
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Platelet Carbonic Anhydrase II, a Forgotten Enzyme, May Be Responsible for Aspirin Resistance
Authors: M Jakubowski, J D?bski, E Szahidewic, A Turek-Jaku, J Gawry?, K Gawry?, R Skomro, A Derkacz, A Doroszko
Oxid Med Cell Longev, 2017;2017(0):3132063.
Species: Human
Sample Types: Plasma
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Plasminogen Activator Inhibitor-1 in depression: Results from Animal and Clinical Studies
Sci Rep, 2016;6(0):30464.
Species: Human
Sample Types: Serum
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Circadian misalignment increases cardiovascular disease risk factors in humans
Proc. Natl. Acad. Sci. U.S.A., 2016;113(10):E1402-11.
Species: Human
Sample Types: Plasma
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MAP3K19 Is a Novel Regulator of TGF-? Signaling That Impacts Bleomycin-Induced Lung Injury and Pulmonary Fibrosis
PLoS ONE, 2016;11(5):e0154874.
Species: Human
Sample Types: Cell Lysates
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Staphylococcal nuclease domain containing-1 (SND1) promotes migration and invasion via angiotensin II type 1 receptor (AT1R) and TGFbeta signaling.
Authors: Santhekadur P, Akiel M, Emdad L, Gredler R, Srivastava J, Rajasekaran D, Robertson C, Mukhopadhyay N, Fisher P, Sarkar D
FEBS Open Bio, 2014;4(0):353-61.
Species: Human
Sample Types: Cell Culture Supernates
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Ghrelin protects against renal damages induced by angiotensin-II via an antioxidative stress mechanism in mice.
Authors: Fujimura K, Wakino S, Minakuchi H, Hasegawa K, Hosoya K, Komatsu M, Kaneko Y, Shinozuka K, Washida N, Kanda T, Tokuyama H, Hayashi K, Itoh H
PLoS ONE, 2014;9(4):e94373.
Species: Human
Sample Types: Cell Culture Supernates
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Characterization of circulating and endothelial progenitor cells in patients with extreme-duration type 1 diabetes.
Authors: Hernandez S, Gong J, Chen L, Wu I, Sun J, Keenan H, King G
Diabetes Care, 2014;37(8):2193-201.
Species: Human
Sample Types: Plasma
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Vitamin D insufficiency and subclinical atherosclerosis in non-diabetic males living with HIV.
Authors: Portilla J, Moreno-Perez O, Serna-Candel C, Escoin C, Alfayate R, Reus S, Merino E, Boix V, Giner L, Sanchez-Paya J, Pico A
J Int AIDS Soc, 2014;17(0):18945.
Species: Human
Sample Types: Serum
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Coinfection with human herpesvirus 8 is associated with persistent inflammation and immune activation in virologically suppressed HIV-infected patients.
Authors: Masia M, Robledano C, Ortiz de la Tabla V, Antequera P, Lumbreras B, Hernandez I, Gutierrez F
PLoS ONE, 2014;9(8):e105442.
Species: Human
Sample Types: Plasma
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Increased metabolite levels of glycolysis and pentose phosphate pathway in rabbit atherosclerotic arteries and hypoxic macrophage.
Authors: Yamashita A, Zhao Y, Matsuura Y, Yamasaki K, Moriguchi-Goto S, Sugita C, Iwakiri T, Okuyama N, Koshimoto C, Kawai K, Tamaki N, Zhao S, Kuge Y, Asada Y
PLoS ONE, 2014;9(1):e86426.
Species: Human
Sample Types: Cell Culture Supernates
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p-Cresol affects reactive oxygen species generation, cell cycle arrest, cytotoxicity and inflammation/atherosclerosis-related modulators production in endothelial cells and mononuclear cells.
Authors: Chang M, Chang H, Chan C, Yeung S, Hsien H, Lin B, Yeh C, Tseng W, Tseng S, Jeng J
PLoS ONE, 2014;9(12):e114446.
Species: Human
Sample Types: Whole Cells
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Dipyridamole decreases inflammatory metalloproteinase-9 expression and release by human monocytes.
Thromb Haemost, 2013;109(2):280-9.
Species: Human
Sample Types: Cell Culture Supernates
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Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis.
BMC Infect Dis, 2012;12(0):144.
Species: Human
Sample Types: Plasma
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Recombinant GPI-anchored TIMP-1 stimulates growth and migration of peritoneal mesothelial cells.
Authors: Djafarzadeh R, Sauter M, Notohamiprodjo S, Noessner E, Goyal P, Siess W, Wornle M, Ribeiro A, Himmelein S, Sitter T, Nelson PJ
PLoS ONE, 2012;7(4):e33963.
Species: Human
Sample Types: Cell Lysates
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Endothelial differentiation of adipose-derived stem cells from elderly patients with cardiovascular disease.
Authors: Zhang P, Moudgill N, Hager E, Tarola N, Dimatteo C, McIlhenny S, Tulenko T, DiMuzio PJ
Stem Cells Dev., 2011;20(6):977-88.
Species: Human
Sample Types: Cell Culture Supernates
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Exercise training improves sleep pattern and metabolic profile in elderly people in a time-dependent manner.
Authors: Lira FS, Pimentel GD, Santos RV, Oyama LM, Damaso AR, Oller do Nascimento CM, Viana VA, Boscolo RA, Grassmann V, Santana MG, Esteves AM, Tufik S, de Mello MT
Lipids Health Dis, 2011;10(0):113.
Species: Human
Sample Types: Plasma
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SPARC suppresses apoptosis of idiopathic pulmonary fibrosis fibroblasts through constitutive activation of beta-catenin.
Authors: Chang W, Wei K, Jacobs SS, Upadhyay D, Weill D, Rosen GD
J. Biol. Chem., 2010;285(11):8196-206.
Species: Human
Sample Types: Cell Culture Supernates
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Cyclic adenosine monophosphate induces plasminogen activator inhibitor-1 expression in human mast cells.
Authors: Ma Z, Kwong KY, Tovar JP, Paek D
Biochem. Biophys. Res. Commun., 2010;400(4):569-74.
Species: Human
Sample Types: Cell Culture Supernates
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Placenta growth factor (PlGF), a novel inducer of plasminogen activator inhibitor-1 (PAI-1) in sickle cell disease (SCD).
Authors: Patel N, Sundaram N, Yang M
J. Biol. Chem., 2010;285(22):16713-22.
Species: Human
Sample Types: Plasma
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Syndromic and non-syndromic aneurysms of the human ascending aorta share activation of the Smad2 pathway.
Authors: Gomez D, Al Haj Zen A, Borges LF, Philippe M, Gutierrez PS, Jondeau G, Michel JB, Vranckx R
J. Pathol., 2009;218(1):131-42.
Species: Human
Sample Types: Cell Culture Supernates
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Urokinase induces survival or pro-apoptotic signals in human mesangial cells depending on the apoptotic stimulus.
Authors: Tkachuk N, Kiyan J, Tkachuk S, Kiyan R, Shushakova N, Haller H, Dumler I
Biochem. J., 2008;415(2):265-73.
Species: Human
Sample Types: Cell Culture Supernates
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Adiponectin regulates albuminuria and podocyte function in mice.
Authors: Sharma K, Ramachandrarao S, Qiu G, Usui HK, Zhu Y, Dunn SR, Ouedraogo R, Hough K, McCue P, Chan L, Falkner B, Goldstein BJ
J. Clin. Invest., 2008;118(5):1645-56.
Species: Human
Sample Types: Plasma
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MSC from fetal and adult lungs possess lung-specific properties compared to bone marrow-derived MSC.
Authors: Rolandsson Enes S, Andersson Sjoland A, Skog I, Hansson L, Larsson H, Le Blanc K, Eriksson L, Bjermer L, Scheding S, Westergren-Thorsson G
Sci Rep, 0;6(0):29160.
Species: Human
Sample Types: Cell Culture Supernates
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Human CD4- 8- T cells are a distinctive immunoregulatory subset.
Authors: Huang MC, Patel K, Taub DD, Longo DL, Goetzl EJ
FASEB J, 0;24(7):2558-66.
Species: Human
Sample Types: Cell Culture Supernates
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Reviews for Human Serpin E1/PAI-1 Quantikine ELISA Kit
Average Rating: 5 (Based on 4 Reviews)
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Standard curve in duplicate in Columns 1 and 2
Comprehensive and accurate assay that was easy to execute
Great assay with a very clear protocol. Produced results as expected.