Rab11A Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-49320

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Rab11A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: RRENDMSPSNNVVPIHVPPTTENKPKVQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Rab11A Antibody - BSA Free (NBP2-49320) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Rab11A Antibody - BSA Free

Western Blot: Rab11A Antibody [NBP2-49320]

Western Blot: Rab11A Antibody [NBP2-49320]

Western Blot: Rab11A Antibody [NBP2-49320] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
Lane 4: Human plasma
Lane 5: Human Liver tissue
Lane 6: Human Tonsil tissue
Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320]

Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320]

Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320] - Staining of human stomach shows moderate cytoplasmic positivity in parietal cells.
Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320]

Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320]

Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320] - Staining of human colon shows moderate positivity in glandular cells.
Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320]

Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320]

Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320]

Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320]

Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Applications for Rab11A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

validated from a verified customer review.

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 2 reviews rated 4 using NBP2-49320 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rab11A

Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There are over 40 Rab proteins in mammals. Rab11a and Rab11b are known markers for protein trafficking, sorting, and recycling in the endosomal pathway. The Rab11 proteins are enriched in recycling endosomes and the trans-Golgi network, where they regulate membrane recycling back to the plasma membrane. Rab11a is ubiquitously expressed, while Rab11b is expressed mainly in the heart and brain.

Long Name

RAs Genes from Brain Protein 11A

Alternate Names

Rab-11, YL8

Gene Symbol

RAB11A

Additional Rab11A Products

Product Documents for Rab11A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Rab11A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epithelial Cell Polarity

Customer Reviews for Rab11A Antibody - BSA Free (2)

4 out of 5
2 Customer Ratings
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • Rab11A Antibody
    Name: Scott Messenger
    Application: Immunocytochemistry
    Sample Tested: MDA MB 231 cells
    Species: Human
    Verified Customer | Posted 03/28/2017
    MDA-MB-231 cells using antibody 1/100
    Rab11A Antibody - BSA Free NBP2-49320
  • beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody (NK/804)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-231 Cell Lysate
    Species: Human
    Verified Customer | Posted 01/20/2017
    Endogenous levels of Rab11 with 50 ug MDA-MB-231 whole cell lysate from Ctrl (scrambled shRNA) or shRNA Rab11a cell.
    strongest band at ~25 kDa non-specific bands found at >50kDa not lost with shRNA knockdown. Blot is dirty and could be cleaned up with better experimental technique.
    Rab11A Antibody - BSA Free NBP2-49320

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Protocols

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FAQs

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