RAB31 Antibody (1C6) - Azide and BSA Free

Novus Biologicals | Catalog # H00011031-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, ELISA

Cited:

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1C6

Format

Azide and BSA Free
View all RAB31 Primary Antibodies »

Product Specifications

Immunogen

RAB31 (AAH01148, 96 a.a. ~ 195 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TLKKWVKELKEHGPENIVMAIAGNKCDLSDIREVPLKDAKEYAESIGAIVVETSAKNAINIEELFQGISRQIPPLDPHENGNNGTIKVEKPTMQASRRCC

Localization

Cell membrane; Lipid-anchor; Cytoplasmic side.

Specificity

RAB31 - RAB31, member RAS oncogene family

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for RAB31 Antibody (1C6) - Azide and BSA Free

Western Blot: RAB31 Antibody (1C6) [H00011031-M01]

Western Blot: RAB31 Antibody (1C6) [H00011031-M01]

RAB31-Antibody-1C6-Western-Blot-H00011031-M01-img0002.jpg
Western Blot: RAB31 Antibody (1C6) [H00011031-M01]

Western Blot: RAB31 Antibody (1C6) [H00011031-M01]

Western Blot: RAB31 Antibody (1C6) [H00011031-M01] - Western Blot detection against Immunogen (36.63 KDa).
RAB31 Antibody (1C6)

Western Blot: RAB31 Antibody (1C6) [H00011031-M01] -

Western Blot: RAB31 Antibody (1C6) [H00011031-M01] - Characterization of polyclonal antibodies directed to human rab31. (A) The reaction pattern of polyclonal antibody (pAb) RT3-IgG (black) & of the pre-immune rabbit serum (gray) was analyzed by a ‘one-sided ELISA’ assay [19]. rab31-His, purified recombinant, histidine-tagged rab31, used for immunization; GST-rab31, purified recombinant GST-rab31 fusion protein; BSA, bovine serum albumin. (B) In Western blot analyses, pAb RT3-IgG strongly reacts with recombinant rab31 & not at all or only weakly with the highly homologous Rab proteins rab5 & rab22A, respectively. (C) Immunohistochemical staining of paraffin-embedded, formalin-fixed breast cancer specimens with pAb RT4-IgG. Specific immunostaining is observed in the cytoplasm as well as in the nucleus of cancer cells (upper panel, see arrow), stromal cells are stained less frequently (lower panel, see arrow). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22920728), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
RAB31 Antibody (1C6)

Western Blot: RAB31 Antibody (1C6) [H00011031-M01] -

Western Blot: RAB31 Antibody (1C6) [H00011031-M01] - Rab31 expression levels in stably transfected breast cancer cells. (A) pAb RT3-IgG specifically detects rab31 in cell lysates from stably transfected MDA-MB-231 & CAMA-1 breast cancer cells in Western blot analyses. Vector control, MDA-MB-231, or CAMA-1 cells were stably transfected with the empty vector pRcRSV; batch, parental MDA-MB-231 (low endogenous rab31 expression) or CAMA-1 (no endogenous rab31 expression) cells were stably transfected with the pRcRSV-based rab31 expression vector; high, medium or low, selected cell clones of batch-transfected MDA-MB-231 or CAMA-1 cells with differing high, medium or low protein levels of rab31. (B) Rab31 antigen values (ng/mg of total protein) in rab31-transfected MDA-MB-231 & CAMA-1 cells, determined by rab31-ELISA. Mean values (± SEM) of at least three independent experiments are depicted. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22920728), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for RAB31 Antibody (1C6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RAB31

RAB31 (RAB31, member RAS oncogene family) is thought to be involved in trans-Golgi network to-endosome trafficking and may have a role in anterograde exit from the (trans-Golgi network) TGN.

Alternate Names

RAB22B, RAB31, member RAS oncogene family, Ras-related protein Rab-22B, ras-related protein Rab-31

Entrez Gene IDs

11031 (Human)

Gene Symbol

RAB31

OMIM

605694 (Human)

UniProt

AAH01148 (Human)

Additional RAB31 Products

Product Documents for RAB31 Antibody (1C6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RAB31 Antibody (1C6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RAB31 Antibody (1C6) - Azide and BSA Free

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