RAB3IP Antibody (OTI5F2)

Novus Biologicals | Catalog # NBP2-45500

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5F2
View all RAB3IP Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RAB3IP (NP_071901) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

51.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RAB3IP Antibody (OTI5F2)

Western Blot: RAB3IP Antibody (OTI5F2) [NBP2-45500]

Western Blot: RAB3IP Antibody (OTI5F2) [NBP2-45500]

Western Blot: RAB3IP Antibody (OTI5F2) [NBP2-45500] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAB3IP.
Immunocytochemistry/ Immunofluorescence: RAB3IP Antibody (OTI5F2) [NBP2-45500]

Immunocytochemistry/ Immunofluorescence: RAB3IP Antibody (OTI5F2) [NBP2-45500]

Immunocytochemistry/Immunofluorescence: RAB3IP Antibody (OTI5F2) [NBP2-45500] - RAB3IP Antibody (5F2) [NBP2-45500] - 3dpf zebrafish neuromast immunostained for Rab3IP (red) and counterstained with phalloidin (green). Image courtesy of customer.
Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500]

Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500]

Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RAB3IP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3min) (1:150)
Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500]

Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500]

Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RAB3IP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3min) (1:150)
Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500]

Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500]

Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RAB3IP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3min) (1:150)
Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500]

Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500]

Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500] - Staining of paraffin-embedded Human colon tissue within the normal limits using anti-RAB3IP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3min) (1:150)

Applications for RAB3IP Antibody (OTI5F2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:150

Western Blot

1:2000

Reviewed Applications

Read 1 review rated 3 using NBP2-45500 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAB3IP

RAB3IP, also known as Rab-3A-interacting protein, is a 53 kDa 476 amino acid protein with 8 isoforms produced by alternative splicing. The primary function of RAB3IP is to change GDP proteins into GTP proteins. Current research on RAB3IP is being conducted in relation to vitelliform, macular dystrophy, pilocytic astrocytoma, anaplastic astrocytoma and astrocytoma. RAB3IP is linked to the vesicle docking, metaphase, exocytosis, mitosis, transport, synaptic transmission and cytokinesis pathways where it interacts with RAB3IL1, SSX2B, SSX2, RAB8A and RAB3A.

Alternate Names

FLJ14660, FLJ22548, MGC71495, RAB3A interacting protein (rabin3), rab-3A-interacting protein, Rab3A-interacting protein, RABIN3, Rabin-3, SSX2 interacting protein, SSX2-interacting protein

Gene Symbol

RAB3IP

Additional RAB3IP Products

Product Documents for RAB3IP Antibody (OTI5F2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RAB3IP Antibody (OTI5F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for RAB3IP Antibody (OTI5F2) (1)

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Customer Images

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  • RAB3IP Antibody (5F2)
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: whole mount zebrafish
    Species: Zebrafish
    Verified Customer | Posted 02/28/2017
    3dpf zebrafish neuromast immunostained for Rab3IP (red) and counterstained with phalloidin (green)
    3-5dpf larvae were fixed at 4⁰C overnight with 4% PFA in PBS and then rinsed twice with PBS + Triton X-100 0.1%. Primary antibody was diluted in PBSTx 0.1% containing 2.5% BSA and 2.5% FBS at 1:200 dilution and samples incubated overnight with rocking at 4⁰C. After several washes larvae were incubated with AlexaFluor 594-mouse conjugated antibody and AlexaFluor 488-phalloidin.
    RAB3IP Antibody (OTI5F2) NBP2-45500

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Protocols

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