RAB9A Antibody (23GB1940)

Novus Biologicals | Catalog # NBP3-50957

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB1940
View all RAB9A Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human RAB9A (Uniprot: P51151)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAB9A Antibody (23GB1940)

RAB9A Antibody (23GB1940)

Immunocytochemistry/ Immunofluorescence: RAB9A Antibody (23GB1940)[NBP3-50957] -

RAB9A antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: Rab9 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Rab9 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um. Permeabilization: Triton.
RAB9A Antibody (23GB1940)

Immunocytochemistry/ Immunofluorescence: RAB9A Antibody (23GB1940)[NBP3-50957] -

Immunocytochemical staining of HepG2 cells with RAB9A antibody (1:1,000). Nuclei were stained blue with DAPI; RAB9A was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
RAB9A Antibody (23GB1940)

Flow Cytometry: RAB9A Antibody (23GB1940)[NBP3-50957] -

Flow cytometric analysis of RAB9Aexpression in HepG2 cells using RAB9A antibody (1:2,000). Green, isotype control; red, Rab9.
RAB9A Antibody (23GB1940)

Flow Cytometry: RAB9A Antibody (23GB1940)[NBP3-50957] -

Validation of RAB9A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with RAB9A antibody (1:2,000) and analyzed using CytoFLEX.
RAB9A Antibody (23GB1940)

Western Blot: RAB9A Antibody (23GB1940)[NBP3-50957] -

Western blotting analysis using RAB9A antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.
RAB9A Antibody (23GB1940)

Western Blot: RAB9A Antibody (23GB1940)[NBP3-50957] -

Western blotting analysis using RAB9A antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for RAB9A Antibody (23GB1940)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAB9A

Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.

Alternate Names

RAB9A, member RAS oncogene family, RAB9RAB9, member RAS oncogene family, ras-related protein Rab-9A

Gene Symbol

RAB9A

Additional RAB9A Products

Product Documents for RAB9A Antibody (23GB1940)

Certificate of Analysis

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Product Specific Notices for RAB9A Antibody (23GB1940)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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