Rad23 Antibody (3B7T9)

Novus Biologicals | Catalog # NBP3-16573

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3B7T9 expressed in HEK293
View all Rad23 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Rad23 (P54725). TGIPGSPEPEHGSVQESQVSEQPATEAAGENPLEFLRDQPQFQNMRQVIQQNPALLPALLQQLGQENPQLLQQISRHQEQFIQMLNEPPGELADISDVEGE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rad23 Antibody (3B7T9)

Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573]

Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573]

Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573] - Western blot analysis of extracts of various cell lines, using Rad23 Rabbit mAb (NBP3-16573) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.
Immunocytochemistry/ Immunofluorescence: Rad23 Antibody (3B7T9) [NBP3-16573]

Immunocytochemistry/ Immunofluorescence: Rad23 Antibody (3B7T9) [NBP3-16573]

Immunocytochemistry/Immunofluorescence: Rad23 Antibody (3B7T9) [NBP3-16573] - Immunofluorescence analysis of U-2 OS cells using Rad23 Rabbit mAb (NBP3-16573) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573]

Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573]

Western Blot: Rad23 Antibody (3B7T9) [NBP3-16573] - Western blot analysis of extracts of various cell lines, using Rad23 Rabbit mAb (NBP3-16573) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: Rad23 Antibody (3B7T9) [NBP3-16573]

Immunocytochemistry/ Immunofluorescence: Rad23 Antibody (3B7T9) [NBP3-16573]

Immunocytochemistry/Immunofluorescence: Rad23 Antibody (3B7T9) [NBP3-16573] - Immunofluorescence analysis of NIH-3T3 cells using Rad23 Rabbit mAb (NBP3-16573) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Rad23 Antibody (3B7T9)

Immunocytochemistry/ Immunofluorescence: Rad23 Antibody (3B7T9) [NBP3-16573] -

Immunocytochemistry/ Immunofluorescence: Rad23 Antibody (3B7T9) [NBP3-16573] - Immunofluorescence analysis of NIH-3T3 cells using Rad23 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for Rad23 Antibody (3B7T9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rad23

Rad23 is an evolutionarily conserved protein that is important for nucleotide excision repair. All eukaryotic Rad23 proteins contain an amino-terminal UbL domain that bears a striking resemblance to ubiquitin. Rad23 has a proteolytic function in DNA repair via its interaction with the 26S proteosome through the UbL domain. The elimination of this Rad23 domain causes sensitivity to ultraviolet radiation.

Alternate Names

hHR23

Gene Symbol

RAD23A

Additional Rad23 Products

Product Documents for Rad23 Antibody (3B7T9)

Certificate of Analysis

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Product Specific Notices for Rad23 Antibody (3B7T9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Base Excision Repair

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Protocols

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FAQs

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