Rad51 is involved in both the recombination and repair of DNA damage. Its function may be modulated by interaction with other repair proteins including BRCA1 and BRCA2. Alterations in the Rad51 gene have been associated with increased cancer risk. Rad51 is conserved across species and is homologous to prokaryotic RecA.
Rad51 Antibody (RAD51/2701) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-79895
![Western Blot: Rad51 Antibody (RAD51/2701)Azide and BSA Free [NBP2-79895]](https://beta-resources.rndsystems.com/images/products/Rad51-Antibody-RAD51-2701-Azide-and-BSA-Free-Western-Blot-NBP2-79895-img0002.jpg "Western Blot: Rad51 Antibody (RAD51/2701)Azide and BSA Free [NBP2-79895]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Protein Array, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # RAD51/2701
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fragment of human Rad51 protein (around aa 1-134) (exact sequence is proprietary) (Uniprot: Q06609)
Localization
Nuclear
Marker
Prognostic and Response to Chemotherapy Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79760).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Rad51 Antibody (RAD51/2701) - Azide and BSA Free
Western Blot: Rad51 Antibody (RAD51/2701)Azide and BSA Free [NBP2-79895]
Western Blot: Rad51 Antibody (RAD51/2701) - Azide and BSA Free [NBP2-79895] - Western Blot Analysis of HeLa, HepG2, K562, A431 and MCF-7 cell lysate using Rad51 Antibody (RAD51/2701).![Flow Cytometry: Rad51 Antibody (RAD51/2701) - Azide and BSA Free [NBP2-79895]](https://beta-resources.rndsystems.com/images/products/Rad51-Antibody-RAD51-2701-Azide-and-BSA-Free-Flow-Cytometry-NBP2-79895-img0003.jpg "Flow Cytometry: Rad51 Antibody (RAD51/2701) - Azide and BSA Free [NBP2-79895]")
Flow Cytometry: Rad51 Antibody (RAD51/2701) - Azide and BSA Free [NBP2-79895]
Flow Cytometry: Rad51 Antibody (RAD51/2701) - Azide and BSA Free [NBP2-79895] - Flow Cytometric Analysis of K562 cells using Rad51 Antibody (RAD51/2701)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).![Protein Array: Rad51 Antibody (RAD51/2701) - Azide and BSA Free [NBP2-79895]](https://beta-resources.rndsystems.com/images/products/Rad51-Antibody-RAD51-2701-Azide-and-BSA-Free-Protein-Array-NBP2-79895-img0001.jpg "Protein Array: Rad51 Antibody (RAD51/2701) - Azide and BSA Free [NBP2-79895]")
Protein Array: Rad51 Antibody (RAD51/2701) - Azide and BSA Free [NBP2-79895]
Protein Array: Rad51 Antibody (RAD51/2701) - Azide and BSA Free [NBP2-79895] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Rad51 Antibody (RAD51/2701) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.Applications for Rad51 Antibody (RAD51/2701) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug/ml
Immunohistochemistry
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Rad51
Alternate Names
BRCC5, hRAD51, HsRad51, HsT16930, RAD51 (S. cerevisiae) homolog (E coli RecA homolog), RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae), RAD51 homolog A, RAD51ABRCA1/BRCA2-containing complex, subunit 5, RAD51L3, RecA, E. coli, homolog of, RECADNA repair protein RAD51 homolog 1, RecA-like protein, recombination protein A
Gene Symbol
RAD51
Additional Rad51 Products
Product Documents for Rad51 Antibody (RAD51/2701) - Azide and BSA Free
Product Specific Notices for Rad51 Antibody (RAD51/2701) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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