RalA/RalB Antibody (3E6) - Azide and BSA Free

Novus Biologicals | Catalog # H00005898-M01

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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3E6

Format

Azide and BSA Free
View all RalA/RalB Primary Antibodies »

Product Specifications

Immunogen

Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S194 of human RALA. The mark "*" on the immunogen sequence is the site of phosphorylation. CNGKKKRKS*LAKRI

Specificity

RALA - v-ral simian leukemia viral oncogene homolog A (ras related) (3E6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein. Mouse monoclonal antibody raised against a partial-length synthetic RALA.

Scientific Data Images for RalA/RalB Antibody (3E6) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: RalA/RalB Antibody (3E6) [H00005898-M01]

Immunocytochemistry/ Immunofluorescence: RalA/RalB Antibody (3E6) [H00005898-M01]

Immunocytochemistry/Immunofluorescence: RalA/RalB Antibody (3E6) [H00005898-M01] - Analysis of monoclonal antibody to RALA on HeLa cell. Antibody concentration 10 ug/ml.
Proximity Ligation Assay: RalA/RalB Antibody (3E6) [H00005898-M01]

Proximity Ligation Assay: RalA/RalB Antibody (3E6) [H00005898-M01]

Proximity Ligation Assay: RalA/RalB Antibody (3E6) [H00005898-M01] - Analysis of protein-protein interactions between RALBP1 and RALA. HeLa cells were stained with anti-RALBP1 rabbit purified polyclonal 1:1200 and anti-RALA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for RalA/RalB Antibody (3E6) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RalA/RalB

The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins.

Long Name

Ras-like Protein A/B

Alternate Names

MGC48949, RAL, Ras family small GTP binding protein RALA, RAS-like protein A, ras-related protein Ral-A, v-ral simian leukemia viral oncogene homolog A (ras related)

Entrez Gene IDs

5898 (Human)

Gene Symbol

RALA

UniProt

NP_005393 (Human)

Additional RalA/RalB Products

Product Documents for RalA/RalB Antibody (3E6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for RalA/RalB Antibody (3E6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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