![Western Blot: RALY Antibody [NBP1-19111]](https://resources.rndsystems.com/images/products/RALY-Antibody-Western-Blot-NBP1-19111-img0003.jpg "Western Blot: RALY Antibody [NBP1-19111]")
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 256 and 306 of human hnRNP-associated with lethal yellow homolog using the numbering given in entry NP_057951.1 (GeneID 22913).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RALY Antibody
Western Blot: RALY Antibody [NBP1-19111]
Western Blot: RALY Antibody [NBP1-19111] - Whole cell lysate from HeLa and 293T cells. RALY was successfully immunoprecipitated by rabbit anti-RALY antibody NBP1-19110.![Immunohistochemistry: RALY Antibody [NBP1-19111]](https://resources.rndsystems.com/images/products/RALY-Antibody-Immunohistochemistry-NBP1-19111-img0004.jpg "Immunohistochemistry: RALY Antibody [NBP1-19111]")
Immunohistochemistry: RALY Antibody [NBP1-19111]
Immunohistochemistry: RALY Antibody [NBP1-19111] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- RALY used at a dilution of 1:1,000 (0.2 ug/ml) and 1:200 (1ug/ml). Detection: DAB![Immunohistochemistry-Paraffin: RALY Antibody [NBP1-19111]](https://resources.rndsystems.com/images/products/RALY-Antibody-Immunohistochemistry-Paraffin-NBP1-19111-img0001.jpg "Immunohistochemistry-Paraffin: RALY Antibody [NBP1-19111]")
Immunohistochemistry-Paraffin: RALY Antibody [NBP1-19111]
Immunohistochemistry-Paraffin: RALY Antibody [NBP1-19111] - FFPE section of human stomach carcinoma. Affinity purified rabbit anti-RALY used at a dilution of 1:500.![Immunohistochemistry-Paraffin: RALY Antibody [NBP1-19111]](https://resources.rndsystems.com/images/products/RALY-Antibody-Immunohistochemistry-Paraffin-NBP1-19111-img0002.jpg "Immunohistochemistry-Paraffin: RALY Antibody [NBP1-19111]")
Immunohistochemistry-Paraffin: RALY Antibody [NBP1-19111]
Immunohistochemistry-Paraffin: RALY Antibody [NBP1-19111] - FFPE section of mouse renal cell carcinoma. Affinity purified rabbit anti-RALY used at a dilution of 1:100.Applications for RALY Antibody
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:2000-1:10000
Application Notes
This antibody is useful for western blot. It is not recommended for immunoprecipitation.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: RALY
Alternate Names
Autoantigen p542, Heterogeneous nuclear ribonucleoprotein C-like 2, hnRNP associated with lethal yellow protein homolog, hnRNP core protein C-like 2, HNRPCL2MGC117312, P542hnRNP-associated with lethal yellow), RNA binding protein (autoantigenic, hnRNP-associated with lethal yellow), RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog(mouse)), RNA-binding protein (autoantigenic), RNA-binding protein Raly
Entrez Gene IDs
22913 (Human)
Gene Symbol
RALY
UniProt
Additional RALY Products
Product Documents for RALY Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RALY Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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