RAMP2 Antibody (OTI4E5)

Novus Biologicals | Catalog # NBP2-01853

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4E5
View all RAMP2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinat protein of human RAMP2(NP_005845) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

15.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse RAMP2 Antibody (OTI4E5) (NBP2-01853) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for RAMP2 Antibody (OTI4E5)

Western Blot: RAMP2 Antibody (OTI4E5) [NBP2-01853]

Western Blot: RAMP2 Antibody (OTI4E5) [NBP2-01853]

Western Blot: RAMP2 Antibody (4E5) [NBP2-01853] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAMP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAMP2.
Immunohistochemistry-Paraffin: RAMP2 Antibody (OTI4E5) [NBP2-01853]

Immunohistochemistry-Paraffin: RAMP2 Antibody (OTI4E5) [NBP2-01853]

Immunohistochemistry-Paraffin: RAMP2 Antibody (4E5) [NBP2-01853] - FFPE gingival tissue from Macaca mulatta was stained with RAMP2 Antibody (4E5) as described in "Other experimental details". Clear membranous and cytoplasmic staining is seen in the epithelium and also in the inflammatory cells infiltrating the stroma. Antibody diluted 1:150. This image was submitted via customer Review.
Flow Cytometry: RAMP2 Antibody (OTI4E5) [NBP2-01853]

Flow Cytometry: RAMP2 Antibody (OTI4E5) [NBP2-01853]

Flow Cytometry: RAMP2 Antibody (4E5) [NBP2-01853] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RAMP2 antibody, and then analyzed by flow cytometry.
Immunohistochemistry-Paraffin: RAMP2 Antibody (OTI4E5) [NBP2-01853]

Immunohistochemistry-Paraffin: RAMP2 Antibody (OTI4E5) [NBP2-01853]

Immunohistochemistry-Paraffin: RAMP2 Antibody (4E5) [NBP2-01853] - Staining of paraffin-embedded Human Kidney tissue using anti-RAMP2 mouse monoclonal antibody.

Applications for RAMP2 Antibody (OTI4E5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:1000

Reviewed Applications

Read 1 review rated 5 using NBP2-01853 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAMP2

The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP2) protein, CRLR functions as an adrenomedullin receptor. The RAMP2 protein is involved in core glycosylation and transportation of adrenomedullin receptor to the cell surface. [provided by RefSeq].

Long Name

Receptor Activity Modifying Protein 2

Alternate Names

calcitonin receptor-like receptor activity modifying protein 2, Calcitonin-receptor-like receptor activity-modifying protein 2, CRLR activity-modifying protein 2, receptor (calcitonin) activity modifying protein 2, receptor (G protein-coupled) activity modifying protein 2, receptor activity modifying protein 2, receptor activity-modifying protein 2, receptor-activity-modifying protein 2

Entrez Gene IDs

10266 (Human)

Gene Symbol

RAMP2

Additional RAMP2 Products

Product Documents for RAMP2 Antibody (OTI4E5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for RAMP2 Antibody (OTI4E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for RAMP2 Antibody (OTI4E5) (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used RAMP2 Antibody (OTI4E5)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • RAMP2 Antibody (4E5)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Gingival tissue
    Species: Macaca mulatta
    Verified Customer | Posted 02/20/2017
    FFPE gingival tissue from Macaca mulatta was stained with RAMP2 Antibody (4E5) as described in "Other experimental details". Clear membranous and cytoplasmic staining is seen in the epithelium and also in the inflammatory cells infiltrating the stroma.
    HIER: Diva Decloaker 5 minutes + 45 minutes cool down Antibody dilution: 1:150 Antibody incubation: Overnight @ 4 deg C DAB: 5 min
    RAMP2 Antibody (OTI4E5) NBP2-01853
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies