RAP1B Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-94183

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Rap1B Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human Rap1B (NP_056461.1). MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDERVVGKEQGQNLARQWNNCAFLESSAKSKINVNEIFYDLVRQINRKTPVPGKARKKSSCQLL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAP1B Antibody - Azide and BSA Free

Western Blot: RAP1B AntibodyAzide and BSA Free [NBP2-94183]

Western Blot: RAP1B AntibodyAzide and BSA Free [NBP2-94183]

Western Blot: RAP1B Antibody [NBP2-94183] - Analysis of extracts of various cell lines, using Rap1B antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 30s.
RAP1B Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: RAP1B Antibody - Azide and BSA Free [NBP2-94183] -

Immunocytochemistry/ Immunofluorescence: RAP1B Antibody - Azide and BSA Free [NBP2-94183] - Immunofluorescence analysis of HeLa cells using RAP1B Rabbit pAb (A12925) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for RAP1B Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rap1B

RAP1B and RAP1A (MIM 179520) belong to a superfamily of RAS (see MIM 190020)-like small GTP-binding proteins involved in cell signaling.[supplied by OMIM]

Long Name

Ras-related Protein 1B

Alternate Names

KREV, RAL1B

Gene Symbol

RAP1B

Additional Rap1B Products

Product Documents for RAP1B Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for RAP1B Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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