RAPGEF2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-06549

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RAPGEF2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 975 and 1025 of human PDZ domain containing guanine nucleotide exchange factor (GEF) 1 (NP_055062.1).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAPGEF2 Antibody - BSA Free

Western Blot: RAPGEF2 Antibody [NBP1-06549]

Western Blot: RAPGEF2 Antibody [NBP1-06549]

Western Blot: RAPGEF2 Antibody [NBP1-06549] - Whole cell lysate (50 ug) from HeLa, Hep-G2, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PDZ-GEF1 antibodyused for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: RAPGEF2 Antibody [NBP1-06549]

Immunohistochemistry-Paraffin: RAPGEF2 Antibody [NBP1-06549]

Immunohistochemistry-Paraffin: RAPGEF2 Antibody [NBP1-06549] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PDZ-GEF1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: RAPGEF2 Antibody [NBP1-06549]

Immunoprecipitation: RAPGEF2 Antibody [NBP1-06549]

Immunoprecipitation: RAPGEF2 Antibody [NBP1-06549] - Detection of human PDZ-GEF1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PDZ-GEF1 antibody NBP1-06549 used for IP at 6 ug per reaction. For blotting immunoprecipitated PDZ-GEF1, NBP1-06549 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for RAPGEF2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:1000 - 1:5000
Application Notes
Epitope retrieval with citrate buffer pH6.0 or Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RAPGEF2

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF2, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM]

Alternate Names

CNrasGEF, KIAA0313, Neural RAP guanine nucleotide exchange protein, nRap GEP, NRAPGEP, PDZ domain containing guanine nucleotide exchange factor (GEF) 1, PDZGEF1, PDZ-GEF1PDZ domain-containing guanine nucleotide exchange factor 1, RA(Ras/Rap1A-associating)-GEF, RA-GEFDKFZP586O1422, Rap guanine nucleotide exchange factor (GEF) 2, rap guanine nucleotide exchange factor 2, Rap-GEP

Entrez Gene IDs

9693 (Human)

Gene Symbol

RAPGEF2

UniProt

Q9Y4G8 (Human)

Additional RAPGEF2 Products

Product Documents for RAPGEF2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RAPGEF2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RAPGEF2 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for RAPGEF2 Antibody - BSA Free

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  • Q: Hello - I'd be interested in using this antibody to detect endogenous rapgef2 in a rat cell line. We've detected this protein pharmacologically and by RT-PCR, so I strongly suspect that protein should be expressed as well. Was wondering (a) has this antibody every been used in rat tissue? and (b) in the right panel of the figure, Rapgef2 was co-IP'ed with another antibody - is this one available? (c) Do you have any idea what the bands at lower MW in the right image may correspond to?

    A: In regards to your inquiries, A) The antibody has never been tested in rat samples as far as I am showing from the lab testing data. B) The other antibody that this one was Co-IP'ed with is one that is no longer available, which is why the catalog number is not on the datasheet. C) Our lab has not been able to characterize the lower molecular weight non-specific bands at this time.

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