RASA4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15089

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human RASA4. The exact sequence is proprietary.

Reactivity Notes

Mouse (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RASA4 Antibody - BSA Free

Western Blot: RASA4 Antibody [NBP2-15089]

Western Blot: RASA4 Antibody [NBP2-15089]

Western Blot: RASA4 Antibody [NBP2-15089] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7. 5% SDS PAGE, antibody diluted at 1:3000.
Immunocytochemistry/ Immunofluorescence: RASA4 Antibody [NBP2-15089]

Immunocytochemistry/ Immunofluorescence: RASA4 Antibody [NBP2-15089]

Immunocytochemistry/Immunofluorescence: RASA4 Antibody [NBP2-15089] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Applications for RASA4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RASA4

This gene encodes a member of the GAP1 family of GTPase-activating proteins that suppresses the Ras/mitogen-activated protein kinase pathway in response to Ca(2+). Stimuli that increase intracellular Ca(2+) levels result in the translocation of this protein to the plasma membrane, where it activates Ras GTPase activity. Consequently, Ras is converted from the active GTP-bound state to the inactive GDP-bound state and no longer activates downstream pathways that regulate gene expression, cell growth, and differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

Ca2+-promoted Ras inactivator, Calcium-promoted Ras inactivator, CAPRIGAPLKIAA0538RASA4B, FLJ59070, Putative Ras GTPase-activating protein 4B, ras GTPase-activating protein 4, RAS p21 protein activator 4MGC131890, RasGAP-activating-like protein 2

Gene Symbol

RASA4

Additional RASA4 Products

Product Documents for RASA4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RASA4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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