Rasgrp1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-94157

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Rasgrp1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 598-797 of human RASGRP1 (NP_005730.2). PVAPTENNTSVGPVSNLCSLGAKDLLHAPEEGPFTFPNGEAVEHGEESKDRTIMLMGVSSQKISLRLKRAVAHKATQTESQPWIGSEGPSGPFVLSSPRKTAQDTLYVLPSPTSPCPSPVLVRKRAFVKWENKDSLIKSKEELRHLRLPTYQELEQEINTLKADNDALKIQLKYAQKKIESLQLEKSNHVLAQMEQGDCS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rasgrp1 Antibody - BSA Free

Western Blot: Rasgrp1 AntibodyBSA Free [NBP2-94157]

Western Blot: Rasgrp1 AntibodyBSA Free [NBP2-94157]

Western Blot: Rasgrp1 Antibody [NBP2-94157] - Analysis of extracts of THP-1 cells, using Rasgrp1 antibody (NBP2-94157) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Rasgrp1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Rasgrp1 Antibody - BSA Free [NBP2-94157] -

Immunocytochemistry/ Immunofluorescence: Rasgrp1 Antibody - BSA Free [NBP2-94157] - Immunofluorescence analysis of NIH/3T3 cells using Rasgrp1 Rabbit pAb (A10495) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Rasgrp1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Rasgrp1 Antibody - BSA Free [NBP2-94157] -

Immunocytochemistry/ Immunofluorescence: Rasgrp1 Antibody - BSA Free [NBP2-94157] - Immunofluorescence analysis of C6 cells using Rasgrp1 Rabbit pAb (A10495) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Rasgrp1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Rasgrp1 Antibody - BSA Free [NBP2-94157] -

Immunocytochemistry/ Immunofluorescence: Rasgrp1 Antibody - BSA Free [NBP2-94157] - Immunofluorescence analysis of A-549 cells using Rasgrp1 Rabbit pAb (A10495) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for Rasgrp1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rasgrp1

The Rasgrp1 gene is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through

Alternate Names

CALDAG-GEFI, CALDAG-GEFII, hRasGRP1, RAS guanyl releasing protein 1 (calcium and DAG-regulated), RAS guanyl-releasing protein 1, RASGRP

Gene Symbol

RASGRP1

Additional Rasgrp1 Products

Product Documents for Rasgrp1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Rasgrp1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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