Rat Anti-Mouse IgG2A APC-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse IgG2A Primary Antibody by Flow Cytometry. Human peripheral blood lymphocytes were stained with A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG2A, filled histogram) or isotype control (Catalog # MAB003, open histogram), or B) Mouse Anti-Human CD8 alpha Monoclonal Antibody (Catalog # MAB1509, Mouse IgG2B, filled histogram) or isotype control (Catalog # MAB003, open histogram), followed by APC-conjugated Rat Anti-Mouse IgG2A Isotype-specific Secondary Antibody (Catalog # F0130). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
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