Detection of Mouse IgG2A Primary Antibodies by Western Blot.
Western blot shows lysates of HepG2 and Jurkat cell lines. PVDF membrane was probed with anti-HSP90 (Catalog # MAB3286, Mouse IgG2B isotype), anti-HSP60 (Catalog # MAB1800, Mouse IgG2B isotype), anti-GAPDH (Catalog # MAB5718,Mouse IgG1 isotype), anti-Peroxiredoxin 6 (Catalog # MAB3490, Mouse IgG3 isotype), and anti-SOD1 (Catalog # MAB3418, Mouse IgG2A isotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG2A (Catalog # MAB0033) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IgG2A isotype antibody at approximately 18kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) but not Rabbit Anti-Mouse IgG2A Monoclonal Antibody (Catalog # MAB0033). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse IgG2A PrimaryAntibody by Anti-Mouse IgG2A-specific Secondary Antibody.
Human PBMC lymphocytes were stained with Mouseanti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100, Mouse IgG1isotype), Mouse anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG2A isotype),or Mouse anti-Human CD8 alpha Monoclonal Antibody (Catalog # MAB1509, Mouse IgG2Bisotype), followed by Rabbit anti-Mouse IgG2A-specific secondaryantibody (Catalog # MAB0033) then PE-conjugated Goat anti-Rabbit secondaryantibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Immunoglobulin G2A Fc
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