Mouse IgG2A Antibody

R&D Systems | Catalog # MAB0033

Recombinant Monoclonal Antibody.
R&D Systems
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 2214A
View all IgG2A Secondary Antibodies »

Product Specifications

Immunogen

Mouse IgG

Specificity

Detects mouse IgG2A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with mouse IgG1, mouse IgG2B, and mouse IgG3 was observed.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse IgG2A Antibody

Detection of Mouse IgG2APrimary Antibodies by Western Blot.

Detection of Mouse IgG2APrimary Antibodies by Western Blot.

Western blot shows lysates of HepG2 and Jurkat cell lines. PVDF membrane was probed with anti-HSP90 (Catalog # MAB3286, Mouse IgG2Bisotype), anti-HSP60 (Catalog # MAB1800, Mouse IgG2Bisotype), anti-GAPDH (Catalog # MAB5718, Mouse IgG1isotype), anti-Peroxiredoxin 6 (Catalog # MAB3490, Mouse IgG3isotype), and anti-SOD1 (Catalog # MAB3418, Mouse IgG2Aisotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG2A(Catalog # MAB0033) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IgG2Aisotype antibody at approximately 18 kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) but not Rabbit Anti-Mouse IgG2AMonoclonal Antibody (Catalog # MAB0033). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse IgG2APrimary Antibody by Anti-Mouse IgG2Bspecific Secondary Antibody.

Detection of Mouse IgG2APrimary Antibody by Anti-Mouse IgG2Bspecific Secondary Antibody.

Human PBMC lymphocytes were stained with Mouse anti-Human CD3e Monoclonal Antibody (Catalog # MAB100, Mouse IgG1isotype), Mouse anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG2Aisotype), or Mouse anti-Human CD8a Monoclonal Antibody (Catalog # MAB1509, Mouse IgG2Bisotype), followed by Rabbit anti-Mouse IgG2A-specific secondary antibody (Catalog # MAB0033) then PE-conjugated Goat anti-Rabbit secondary antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse IgG2A Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Designed as a secondary antibody to detect cells stained with a primary antibody of mouse IgG2A isotype

Western Blot

0.5 µg/mL
Sample: Mouse IgG2A Isotype Primary Antibodies

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2A Fc

Additional IgG2A Products

Product Documents for Mouse IgG2A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IgG2A Antibody

For research use only

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