Key Product Details

Validated by

Biological Validation

Species Reactivity

Mouse

Applications

Control

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 20102
View all IgG2A Isotype Controls »

Product Specifications

Immunogen

KLH

Specificity

Selected for use as mouse IgG2A isotype control. In direct ELISAs, does not cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rmBLC, rmC10, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rrCINC-3, rhCK beta 8-1, recombinant viral (rv) CMV UL146 T23, rmCRG-2, rhCXC-X, rhDendrokine, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin-2, rmEotaxin-2, rhEotaxin-3, rhFractalkine, rmFractalkine, rrFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhHCC-4, rhI-309, rhIL-8, recombinant porcine IL-8, rhIP-10, rhI-TAC, rmJE, rmKC, rhLeukotactin-1, rhLymphotactin, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-2, rhMCP-3, rhMCP-4, rmMCP-5, rvMCV Type 2, rhMDC, rmMDC, rhMIG, rmMIG, rhMIP-1 alpha, rmMIP-1 alpha, rvMIP-1 alpha, rhMIP-1 beta, rmMIP-1 beta, rvMIP-1 beta, rmMIP-1 gamma, rmMIP-1 delta, rvMIP-1 gamma, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 beta, rhMPIF-1, rhNAP-2, rhParc, rhRANTES, rmRANTES, rhSDF-1 alpha, rmSDF-1 alpha, rhSDF-1 beta, rhTARC, rmTARC, rmTCA-3, rhTeck, or rmTeck.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse IgG2A Isotype Control

Detection of Mouse IgG Isotype Control by Flow Cytometry

Detection of Mouse IgG Control by Flow Cytometry

Human peripheral blood granulocytes were stained with Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Detection of Mouse IgG2a by Immunohistochemistry

Detection of Mouse IgG2a by Immunohistochemistry

Transmammary transfer of BMP9 and BMP10 blocking Abs leads to abnormal hypervascularization in neonatal retinas.(A–C”) Representative images of fluorescent isolectin B4-stained retinas either from P6 neonates fed for 3 days by dams injected on P3 with PBS (A), control IgG2a/b Abs (B), or BMP9/10 blocking Abs (C–C”). (C’,C”) are higher magnification images of the relevant boxed areas in C. a, artery; v, vein; scale bars, 500 μm. (D–L) Higher magnification showing retinal vasculature fields between an artery and a vein (Plexus, D–F), or at the front of an artery (G–I) or a vein (J–L) from neonates treated as in (A–C). Scale bars, 100 μm. (M–O) Scatter plots showing the vascular density on retinal ‘petals’ at the plexus (M), artery front (N), or vein front (O). Data represent mean ± s.e.m. (n = 6 pups per group from 2 dams); ****P < 0.0001, ANOVA and Kruskal-Wallis test. Arrows indicate AVMs, defined as direct shunts between an artery and a vein. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep37366), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse IgG2A Isotype Control

Application
Recommended Usage

Control

Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.

Reviewed Applications

Read 8 reviews rated 4.4 using MAB003 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2A Fc

Additional IgG2A Products

Product Documents for Mouse IgG2A Isotype Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IgG2A Isotype Control

For research use only

Citations for Mouse IgG2A Isotype Control

Customer Reviews for Mouse IgG2A Isotype Control (8)

4.4 out of 5
8 Customer Ratings
5 Stars
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4 Stars
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Customer Images

Showing  1 - 5 of 8 reviews Showing All
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  • Mouse IgG2A Isotype Control
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: LAD2 human mast cells
    Species: Human
    Verified Customer | Posted 04/07/2022
    Mouse IgG2A Isotype Control MAB003
  • Mouse IgG2A Isotype Control
    Name: Abby Sukarto
    Application: Flow Cytometry
    Sample Tested: Bone Extracts
    Species: Human
    Verified Customer | Posted 02/13/2020
    Mouse IgG2A Isotype Control MAB003
  • Mouse IgG2A Isotype Control
    Name: Anonymous
    Application: Block/Neutralize CONTROL
    Sample Tested: Peripheral blood T cells
    Species: Human
    Verified Customer | Posted 01/08/2020
  • Mouse IgG2A Isotype Control
    Name: Abby Sukarto
    Sample Tested: Bone Extracts
    Species: Human
    Verified Customer | Posted 09/10/2019
    Need optimization of your specific cell types and use the same amount as the primary antibody. Saw lots of binding for the isotype control.
  • Mouse IgG2A Isotype Control
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Recombinant protein
    Species: Mouse
    Verified Customer | Posted 08/04/2019
  • Mouse IgG2A Isotype Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 03/23/2018
  • Mouse IgG2A Isotype Control
    Name: Anonymous
    Verified Customer | Posted 06/13/2017
    Control for ICC on dog cells.
    Mouse IgG2A Isotype Control MAB003
  • Mouse IgG2A Isotype Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: NCI-H146
    Species: Human
    Verified Customer | Posted 08/03/2016
    NCI-H146 cells stained with test antibody (blue) or isotype control (red) at 10 ug/ml and then stained with secondary PE antibody.
    Mouse IgG2A Isotype Control MAB003

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