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Mouse IgG2A Isotype Control

MAB003 41 Citations 6 Reviews Datasheet / COA / SDS
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MAB003
Detection of Mouse IgG Control by Flow Cytometry
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Product Details
Citations (41)
FAQs
Supplemental Products
Reviews (6)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IgG2A Isotype Control Summary

Specificity
Selected for use as mouse IgG2A isotype control. In direct ELISAs, does not cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rmBLC, rmC10, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rrCINC-3, rhCK beta 8-1, recombinant viral (rv) CMV UL146 T23, rmCRG-2, rhCXC-X, rhDendrokine, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin-2, rmEotaxin-2, rhEotaxin-3, rhFractalkine, rmFractalkine, rrFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhHCC-4, rhI-309, rhIL-8, recombinant porcine IL-8, rhIP-10, rhI-TAC, rmJE, rmKC, rhLeukotactin-1, rhLymphotactin, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-2, rhMCP-3, rhMCP-4, rmMCP-5, rvMCV Type 2, rhMDC, rmMDC, rhMIG, rmMIG, rhMIP-1 alpha, rmMIP-1 alpha, rvMIP-1 alpha, rhMIP-1 beta, rmMIP-1 beta, rvMIP-1 beta, rmMIP-1 gamma, rmMIP-1δ, rvMIP-1 gamma, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 beta, rhMPIF-1, rhNAP-2, rhParc, rhRANTES, rmRANTES, rhSDF-1 alpha, rmSDF-1 alpha, rhSDF-1 beta, rhTARC, rmTARC, rmTCA-3, rhTeck, or rmTeck.
Source
Monoclonal Mouse IgG2A Clone # 20102
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Control Detection of Mouse IgG Control by Flow Cytometry View Larger

Detection of Mouse IgG Control by Flow Cytometry Human peripheral blood granulocytes were stained with Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names
IgG2A

Product Datasheets

Product Datasheet
COA
SDS

Citations for Mouse IgG2A Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

41 Citations: Showing 1 - 10
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  1. MUC20 expression marks the receptive phase of the human endometrium
    Authors: A Stepanjuk, M Koel, M Pook, M Saare, K Jääger, M Peters, K Krjutškov, S Ingerpuu, A Salumets
    Reprod. Biomed. Online, 2019;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. Statins can suppress dendritic cell-mediated Th2 responses through the repression of OX40-ligand and CCL17 expression
    Authors: N Inagaki-Ka, T Ito, M Inaba, Y Azuma, A Tanaka, PTX Vien, K Kibata, A Satake, S Nomura
    Eur. J. Immunol., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Isotype Control
  3. Function and Immunogenicity of Gene-corrected iPSC-derived Hepatocyte-Like Cells in Restoring Low Density Lipoprotein Uptake in Homozygous Familial Hypercholesterolemia
    Authors: H Okada, C Nakanishi, S Yoshida, M Shimojima, J Yokawa, M Mori, H Tada, T Yoshimuta, K Hayashi, T Yamano, R Hanayama, M Yamagishi, MA Kawashiri
    Sci Rep, 2019;9(1):4695.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Antibody opsonization enhances MAIT cell responsiveness to bacteria via a TNF dependent mechanism
    Authors: Z Bánki, L Krabbendam, D Klaver, T Leng, S Kruis, H Mehta, B Müllauer, D Orth-Hölle, H Stoiber, CB Willberg, P Klenerman
    Immunol. Cell Biol., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration
    Authors: SM Koura, M Salama, M El-Hussiny, MEA Khalil, A Lotfy, SA Hassan, SA Gad Elhak, MA Sobh
    Mol Med Rep, 2019;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Molecular Correlates ofIn VitroResponses to Dacomitinib and Afatinib in Bladder Cancer
    Authors: S Tamura, Y Wang, B Veeneman, D Hovelson, A Bankhead, LJ Broses, G Lorenzatti, M Liebert, JR Rubin, KC Day, M Hussain, N Neamati, S Tomlins, PL Palmbos, P Grivas, ML Day
    Bladder Cancer, 2018;4(1):77-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Pigeon paramyxovirus type 1 from a fatal human case induces pneumonia in experimentally infected cynomolgus macaques (Macaca fascicularis)
    Authors: T Kuiken, P Buijs, P van Run, G van Ameron, M Koopmans, B van den Ho
    Vet. Res., 2017;48(1):80.
    Species: Primate
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Innate activation of human primary epithelial cells broadens the host response to Mycobacterium tuberculosis in the airways
    Authors: AK Reuschl, MR Edwards, R Parker, DW Connell, L Hoang, A Halliday, H Jarvis, N Siddiqui, C Wright, S Bremang, SM Newton, P Beverley, RJ Shattock, OM Kon, A Lalvani
    PLoS Pathog., 2017;13(9):e1006577.
    Applications: Neutralization Control
  9. Phenotypic Differences between Asian and African Lineage Zika Viruses in Human Neural Progenitor Cells
    Authors: F Anfasa, JY Siegers, M van der Kr, N Mumtaz, V Stalin Raj, FMS de Vrij, W Widagdo, G Gabriel, S Salinas, Y Simonin, C Reusken, SA Kushner, MPG Koopmans, B Haagmans, BEE Martina, D van Riel
    mSphere, 2017;2(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. MiR-205/YAP1 in Activated Fibroblasts of Breast Tumor Promotes VEGF-independent Angiogenesis through STAT3 Signaling
    Authors: YE Du, G Tu, G Yang, G Li, D Yang, L Lang, L Xi, K Sun, Y Chen, K Shu, H Liao, M Liu, Y Hou
    Theranostics, 2017;7(16):3972-3988.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  11. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses
    Authors: Wan-Chien Cheng
    Eur J Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  12. Expression of lectin-like transcript-1 in human tissues
    Authors: A Llibre, L Garner, A Partridge, GJ Freeman, P Klenerman, CB Willberg
    F1000Res, 2016;5(0):2929.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  13. EphA4 may contribute to microvessel remodeling in the hippocampal CA1 and CA3 areas in a mouse model of temporal lobe epilepsy
    Mol Med Rep, 2016;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  14. USP6 oncogene promotes Wnt signaling by deubiquitylating Frizzleds
    Proc Natl Acad Sci USA, 2016;113(21):E2945-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10
    Sci Rep, 2016;5(0):37366.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  16. NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure.
    Authors: Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D
    PLoS ONE, 2015;10(8):e0134568.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  17. A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.
    Authors: Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C
    J Immunol, 2014;192(11):5332-42.
    Species: Human
    Sample Types: Whole Blood
    Applications: Isotype Control
  18. Spontaneous CNV in a novel mutant mouse is associated with early VEGF-A-driven angiogenesis and late-stage focal edema, neural cell loss, and dysfunction.
    Authors: Nagai, Norihiro, Lundh von Leithner, Pete, Izumi-Nagai, Kanako, Hosking, Brett, Chang, Bo, Hurd, Ron, Adamson, Peter, Adamis, Anthony, Foxton, Richard, Ng, Yin Shan, Shima, David T
    Invest Ophthalmol Vis Sci, 2014;55(6):3709-19.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Isotype Control
  19. Type I interferons directly inhibit regulatory T cells to allow optimal antiviral T cell responses during acute LCMV infection.
    Authors: Srivastava, Shivani, Koch, Meghan A, Pepper, Marion, Campbell, Daniel J
    J Exp Med, 2014;211(5):961-74.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Rapid target gene validation in complex cancer mouse models using re-derived embryonic stem cells.
    Authors: Huijbers I, Bin Ali R, Pritchard C, Cozijnsen M, Kwon M, Proost N, Song J, de Vries H, Badhai J, Sutherland K, Krimpenfort P, Michalak E, Jonkers J, Berns A
    EMBO Mol Med, 2014;6(2):212-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Tetherin can restrict cell-free and cell-cell transmission of HIV from primary macrophages to T cells.
    Authors: Giese, Sebastia, Marsh, Mark
    PLoS Pathog, 2014;10(7):e1004189.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Leukocyte attraction by CCL20 and its receptor CCR6 in humans and mice with pneumococcal meningitis.
    Authors: Klein M, Brouwer M, Angele B, Geldhoff M, Marquez G, Varona R, Hacker G, Schmetzer H, Hacker H, Hammerschmidt S, van der Ende A, Pfister H, van de Beek D, Koedel U
    PLoS ONE, 2014;9(4):e93057.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  23. Hepatitis C virus pathogen associated molecular pattern (PAMP) triggers production of lambda-interferons by human plasmacytoid dendritic cells.
    Authors: Stone, Amy E L, Giugliano, Silvia, Schnell, Gretja, Cheng, Linling, Leahy, Katelyn, Golden-Mason, Lucy, Gale, Michael, Rosen, Hugo R
    PLoS Pathog, 2013;9(4):e1003316.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization Control
  24. Mesothelial cells give rise to hepatic stellate cells and myofibroblasts via mesothelial-mesenchymal transition in liver injury.
    Proc Natl Acad Sci U S A, 2013;110(6):2324-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  25. Propagation of human prostate cancer stem-like cells occurs through EGFR-mediated ERK activation.
    Authors: Rybak A, Ingram A, Tang D
    PLoS ONE, 2013;8(4):e61716.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  26. Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
    Authors: Nielsen N, Odum N, Urso B, Lanier LL, Spee P
    PLoS ONE, 2012;7(2):e31959.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  27. IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival.
    Authors: Benito-Miguel M, Garcia-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibanez T, Bonilla-Hernan MG, De Ayala CP, Sanchez-Mateos P, Martin-Mola E, Miranda-Carus ME
    PLoS ONE, 2012;7(7):e40620.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  28. Reciprocal expression of IL-25 and IL-17A is important for allergic airways hyperreactivity.
    Authors: Barlow JL, Flynn RJ, Ballantyne SJ, McKenzie AN
    Clin. Exp. Allergy, 2011;41(10):1447-55.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  29. Inhibition of transcription factor specificity protein 1 alters the gene expression profile of keratinocytes leading to upregulation of kallikrein-related peptidases and thymic stromal lymphopoietin.
    Authors: Bin L, Kim BE, Hall CF
    J. Invest. Dermatol., 2011;131(11):2213-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  30. GAS6/Mer axis regulates the homing and survival of the E2A/PBX1-positive B-cell precursor acute lymphoblastic leukemia in the bone marrow niche.
    Authors: Shiozawa Y, Pedersen EA, Taichman RS
    Exp. Hematol., 2010;38(2):132-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  31. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
    Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
    Eur. J. Haematol., 2010;84(3):239-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  32. Autoimmunity in dry eye is due to resistance of Th17 to Treg suppression.
    Authors: Chauhan SK, El Annan J, Ecoiffier T, Goyal S, Zhang Q, Saban DR, Dana R
    J. Immunol., 2009;182(3):1247-52.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  33. TLR7 Agonist 852A Inhibition of Tumor Cell Proliferation Is Dependent on Plasmacytoid Dendritic Cells and Type I IFN.
    Authors: Inglefield JR, Dumitru CD, Alkan SS, Gibson SJ, Lipson KE, Tomai MA, Larson CJ, Vasilakos JP
    J. Interferon Cytokine Res., 2008;28(4):253-63.
  34. Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
    Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
    Blood, 2007;110(13):4319-30.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Control
  35. Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling.
    Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M
    J. Clin. Invest., 2007;117(12):3664-3672.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  36. Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells.
    Authors: Arnold R, Neumann M, Konig W
    Immunology, 2007;121(1):71-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  37. Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation.
    Authors: Smith C, Damas JK, Otterdal K, ØIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P
    J. Am. Coll. Cardiol., 2006;48(8):1591-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  38. Hyperoxia causes angiopoietin 2-mediated acute lung injury and necrotic cell death.
    Authors: Bhandari V, Choo-Wing R, Lee CG, Zhu Z, Nedrelow JH, Chupp GL, Zhang X, Matthay MA, Ware LB, Homer RJ, Lee PJ, Geick A, de Fougerolles AR, Elias JA
    Nat. Med., 2006;12(11):1286-93.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Control
  39. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
  40. LPS/CD14 activation triggers SGLT-1-mediated glucose uptake and cell rescue in intestinal epithelial cells via early apoptotic signals upstream of caspase-3.
    Authors: Yu LC, Turner JR, Buret AG
    Exp. Cell Res., 2006;312(17):3276-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  41. Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2.
    Authors: Traves SL, Smith SJ, Barnes PJ, Donnelly LE
    J. Leukoc. Biol., 2004;76(2):441-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control

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Reviews for Mouse IgG2A Isotype Control

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Mouse IgG2A Isotype Control
By Anonymous on 02/13/2020
Application: Flow Sample Tested: Bone Extracts Species: Human
Flow Cytometry Mouse IgG2A Isotype Control MAB003
Mouse IgG2A Isotype Control
By Anonymous on 01/08/2020
Application: Block/Neutralize CONTROL Sample Tested: Peripheral blood T cells Species: Human
Mouse IgG2A Isotype Control
By Anonymous on 09/10/2019
Application: Other Sample Tested: Bone Extracts Species: Human

Need optimization of your specific cell types and use the same amount as the primary antibody. Saw lots of binding for the isotype control.

Mouse IgG2A Isotype Control
By Anonymous on 08/04/2019
Application: WB Sample Tested: Recombinant protein Species: Mouse
Mouse IgG2A Isotype Control
By Anonymous on 03/23/2018
Application: Flow Sample Tested: Blood mononuclear cells (PBMCs) Species: Human
Mouse IgG2A Isotype Control
By Anonymous on 08/03/2016
Application: Flow Sample Tested: NCI-H146 Species: Human

NCI-H146 cells stained with test antibody (blue) or isotype control (red) at 10 ug/ml and then stained with secondary PE antibody.

Flow Cytometry Mouse IgG2A Isotype Control MAB003