Rat CCR2 Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB8368V-025UG
FAB8368V-100UG
Detection  of CCR2 in HEK293 Human Cell Line Transfected with Rat CCR2 and eGFP by Flow  Cytometry.
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Rat CCR2 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Rat
Specificity
Stains rat CCR2 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2B Clone # 890231
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with rat CCR2
Accession # O55193
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405

Applications

Recommended Concentration
Sample
Flow Cytometry
1.0 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of CCR2 in HEK293 Human Cell Line Transfected with Rat CCR2 and eGFP by Flow  Cytometry. View Larger

Detection of CCR2 in HEK293 Human Cell Line Transfected with Rat CCR2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) rat CCR2 or (B) irrelevant transfectants and eGFP was stained with 1.0 μg Mouse Anti-Rat CCR2 Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB8368V). Quadrant markers were set based on control antibody staining (Catalog # IC0041V). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR2

CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Two alternate splice forms (CCR2A and CCR2B) differ only by the addition of 14 amino acids to the intracellular carboxyl terminal. Rat CCR2 shares 95% aa sequence identity with mouse CCR2.

Entrez Gene IDs
729230 (Human); 12772 (Mouse); 60463 (Rat)
Alternate Names
C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2CKR2B; CCR2; CCR-2; CCR2A; CCR2B; CD192 antigen; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CMKBR2MGC111760; FLJ78302; MCP-1 receptor; MCP-1-RMGC103828; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor

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