Detection of CCR2 in HEK293 Human Cell Line Transfected with Rat CCR2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with rat CCR2 and eGFP were stained with Mouse Anti-Rat CCR2 Monoclonal Antibody (Catalog # MAB8368) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CCR2, also known as CD192, is a 38 kDa 7TM chemokine
receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by
multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and
smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T
cell recruitment during inflammation. Two alternate splice forms (CCR2A and
CCR2B) differ only by the addition of 14 amino acids to the intracellular
carboxyl terminal. Rat CCR2 shares 95% aa sequence identity with mouse CCR2.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.