Detects rat IL-7 R alpha /CD127 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse IL-7 R alpha and less than 10% cross-reactivity with recombinant human IL-7 R alpha is observed.
Polyclonal Sheep IgG
Mouse myeloma cell line NS0-derived recombinant rat IL-7 R alpha /CD127 Glu21-Asp239 Accession # NP_001099888
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of IL‑7 R alpha /CD127 in Rat Splenocytes by Flow Cytometry.
Rat splenocytes were stained with anti-rat CD4 APC‑conjugated antibody and either (A) Sheep Anti-Rat IL‑7 R alpha /CD127 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5607P) or (B) Normal Sheep IgG Phycoerythrin Control (Catalog # IC016P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-7 R alpha/CD127
IL-7 R alpha (IL-7 Receptor alpha; also known as CD127) is a 60-70 kDa member of the type I cytokine receptor family of molecules. It is expressed on resting naïve and memory CD8+ T cells, DN thymocytes, pre-B cells, Th1 CD4+ T cells, dendritic cells and monocytes. IL-7 R alpha heterodimerizes with both the gamma c chain to create the IL-7 receptor, and the TSLPR subunit to generate the TSLP receptor. IL-7 R alpha participates in T cell differentiation, naïve T cell survival, and CD4+ thymocyte proliferation. Mature rat IL-7 R alpha is a 437 amino acid (aa) type I transmembrane glycoprotein. It contains a 219 aa extracellular region (aa 21-239) that possesses one type III fibronectin domain (aa 129-222), and a 193 aa cytoplasmic tail that shows a key phosphorylation site at Tyr447. Over aa 21-239, rat IL-7 R alpha shares 79% and 67% aa identity with mouse and human IL-7 R alpha, respectively.
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