Detection of mGluR1 C-Terminus by Western Blot Western blot of HEK 293 cells expressing mGluR1a and mGluR5 showing the specific immunolabeling of the ~125 kDa monomer and the ~250 kDa dimer of mGluR1a.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Rat metabotropic glutamate receptor 1 (mGluR1a; also mGlu1a) is a 140 kDa, 1181 amino acid (aa), 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. Group I receptors include mGluR1 and mGluR5. Both are postsynaptic, associated with Gq-like proteins, mobilize Ca++ from intracellular stores, and regulate neuronal excitability by influencing ion channel activity. Their activities are not necessarily redundant. When co-expressed on select neurons, mGluR5 tends to dampen mGluR1 activity. On T cells, mGluR1 is inducible and promotes cell proliferation, while mGluR5 is constitutive and blocks proliferation. mGluR1 has a large glycosylated extracellular domain (ECD) of 574 aa. The ECD either covalently homodimerizes or heterodimerizes with the Ca++-sensor receptor (CaSR). Dimerization creates two subunit-linked “open clamshells” which first bind glutamate, then close, and subsequently undergo rearrangement for signal transduction. There are two alternate splice forms of rat mGluR1. Each shows a short aa substitution and premature truncation after N887 in the cytoplasmic region. The cytoplasmic, C-terminal 310 amino acids of rat mGluR1a are 90% and 97% aa identical to the analogous amino acids in human and mouse mGluR1a, respectively.
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