Rat mGluR1 C-Terminus Antibody

  
  • Species Reactivity
    Rat
  • Specificity
    Rat ~125 kDa and ~250 kDa dimer mGluR1a
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Peptide from the C-terminus region of rat mGluR1a
  • Formulation
    100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
  • Immunohistochemistry
    (fozen sections; unpublished observations)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of mGluR1 C-Terminus by Western Blot Western blot of HEK 293 cells expressing mGluR1a and mGluR5 showing the specific immunolabeling of the ~125 kDa monomer and the ~250 kDa dimer of mGluR1a.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: mGluR1
Rat metabotropic glutamate receptor 1 (mGluR1a; also mGlu1a) is a 140 kDa, 1181 amino acid (aa), 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. Group I receptors include mGluR1 and mGluR5. Both are postsynaptic, associated with Gq-like proteins, mobilize Ca++ from intracellular stores, and regulate neuronal excitability by influencing ion channel activity. Their activities are not necessarily redundant. When co-expressed on select neurons, mGluR5 tends to dampen mGluR1 activity. On T cells, mGluR1 is inducible and promotes cell proliferation, while mGluR5 is constitutive and blocks proliferation. mGluR1 has a large glycosylated extracellular domain (ECD) of 574 aa. The ECD either covalently homodimerizes or heterodimerizes with the Ca++-sensor receptor (CaSR). Dimerization creates two subunit-linked “open clamshells” which first bind glutamate, then close, and subsequently undergo rearrangement for signal transduction. There are two alternate splice forms of rat mGluR1. Each shows a short aa substitution and premature truncation after N887 in the cytoplasmic region. The cytoplasmic, C-terminal 310 amino acids of rat mGluR1a are 90% and 97% aa identical to the analogous amino acids in human and mouse mGluR1a, respectively.
  • References:
    1. Pin, J-P. and F. Acher (2002) Curr. Drug Targets CNS Neurol. Disord. 1:297.
    2. Boldyrev, A.A. et al. (2005) J. Neurochem. 95:913.
    3. Poisik, O.V. et al. (2003) J. Neurosci. 23:122.
    4. Ferraguti, F. and R. Shigemoto (2006) Cell Tissue Res. 326:483.
    5. Gama, L. et al. (2001) J. Biol. Chem. 276:39053.
  • Long Name:
    Metabotropic Glutamate Receptor 1
  • Entrez Gene IDs:
    2911 (Human); 14816 (Mouse); 24414 (Rat)
  • Alternate Names:
    glutamate receptor, metabotropic 1; GPRC1A; GRM1; GRM1A; metabotropic glutamate receptor 1; mGluR1; MGLUR1A; MGLUR1GPRC1AmGlu1
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