Rattin Antibody - BSA Free
Novus Biologicals | Catalog # NB300-246
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human, Rat
Applications
Validated:
Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A synthetic peptide mapping to residues 20-35 [VKRLESPNKTRRPYGA] of the Rattin protein.
Reactivity Notes
This antibody reacts with the rat protein. Use in Human reported in scientific literature (PMID:32444831).
Specificity
Reacts with residues 20-35 [VKRLESPNKTRRPYGA] of the Rattin peptide, which is not in the conserved region to Humanin.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Rattin Antibody - BSA Free
Western Blot: Rattin AntibodyBSA Free [NB300-246]
Western Blot: Rattin Antibody [NB300-246] - Analysis of Rattin using NB300-246 using 100 ng of Rattin peptide and from 50 ug of total lysates of 293 T cells transfected with plasmids encoding GFP-Rattin.Western Blot: Rattin Antibody - BSA Free [NB300-246] -
HNG decreases Rattin expression in gentamicin-exposed cochlear hair cells.The expression of Rattin in OC explants was measured by real-time PCR. OC explants were treated with 200 μM gentamicin (GM) in combination with or without 1 uM HNG (A) or 0.1 uM SHLP3 (B) for 6 h and 16 h. At least three explants were used as sample pool for each condition. Values are presented as mean+S.D. *P < 0.05, ns no significant between all groups. C Western blot results showing the expression of RATTIN after 24-h peptide treatment (1 uM HNG or 0.08 uM SHLP3). At least six explants were used as a sample pool for each condition. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39433756), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Rattin Antibody - BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID:32444831).Western blot The investigator should determine the optimal dilution for a specific application such as immunohistochemistry.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Rattin
Alternate Names
Humanin-like in rat, rattin humanin-like
Additional Rattin Products
Product Documents for Rattin Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Rattin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Rattin Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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