Rattin Antibody - BSA Free

Novus Biologicals | Catalog # NB300-246

Novus Biologicals
Loading...

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
Loading...

Product Specifications

Immunogen

A synthetic peptide mapping to residues 20-35 [VKRLESPNKTRRPYGA] of the Rattin protein.

Reactivity Notes

This antibody reacts with the rat protein. Use in Human reported in scientific literature (PMID:32444831).

Specificity

Reacts with residues 20-35 [VKRLESPNKTRRPYGA] of the Rattin peptide, which is not in the conserved region to Humanin.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rattin Antibody - BSA Free

Western Blot: Rattin AntibodyBSA Free [NB300-246]

Western Blot: Rattin AntibodyBSA Free [NB300-246]

Western Blot: Rattin Antibody [NB300-246] - Analysis of Rattin using NB300-246 using 100 ng of Rattin peptide and from 50 ug of total lysates of 293 T cells transfected with plasmids encoding GFP-Rattin.
Rattin Antibody - BSA Free

Western Blot: Rattin Antibody - BSA Free [NB300-246] -

HNG decreases Rattin expression in gentamicin-exposed cochlear hair cells.The expression of Rattin in OC explants was measured by real-time PCR. OC explants were treated with 200 μM gentamicin (GM) in combination with or without 1 uM HNG (A) or 0.1 uM SHLP3 (B) for 6 h and 16 h. At least three explants were used as sample pool for each condition. Values are presented as mean+S.D. *P < 0.05, ns no significant between all groups. C Western blot results showing the expression of RATTIN after 24-h peptide treatment (1 uM HNG or 0.08 uM SHLP3). At least six explants were used as a sample pool for each condition. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39433756), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Rattin Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID:32444831).Western blot The investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rattin

A novel rat gene, Rattin, encodes a peptide homologous to Humanin, a secreted peptide that specifically protects against neuronal cell death induced by beta-amyloid peptide (Ab) or by mutations causing early-onset familial Alzheimer's disease. Rattin is the prototype of a novel class of peptides, phylogenetically related to Humanin, endowed with protective activity not only against Ab but also toward excitotoxic neuronal death. The rat gene, Rattin, encodes a peptide of 38 residues (15 residues longer than Humanin) showing 73% identity in the conserved region to Humanin.

Alternate Names

Humanin-like in rat, rattin humanin-like

Additional Rattin Products

Product Documents for Rattin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rattin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Rattin Antibody - BSA Free

Customer Reviews for Rattin Antibody - BSA Free

There are currently no reviews for this product. Be the first to review Rattin Antibody - BSA Free and earn rewards!

Have you used Rattin Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies
Loading...