RB1 Antibody (1F8) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-54483

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1F8

Format

Azide and BSA Free
View all RB1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human RB1 protein fragment of 283 amino acid residues. (Uniprot: P06400)

Epitope

aa 703-772

Localization

Nuclear

Specificity

Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53398).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for RB1 Antibody (1F8) - Azide and BSA Free

Immunohistochemistry-Paraffin: RB1 Antibody (1F8) - Azide and BSA Free [NBP2-54483]

Immunohistochemistry-Paraffin: RB1 Antibody (1F8) - Azide and BSA Free [NBP2-54483]

Immunohistochemistry-Paraffin: RB1 Antibody (1F8) - Azide and BSA Free [NBP2-54483] - Formalin-fixed, paraffin-embedded human Colon stained with RB1 Antibody (1F8).

Applications for RB1 Antibody (1F8) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1 - 2 ug/ml

Immunoprecipitation

0.5 - 1 ug

Western Blot

0.5 - 1 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: RB1

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of ts function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

Long Name

Retinoblastoma-associated protein

Alternate Names

OSRC, p105-Rb, pp110

Gene Symbol

RB1

Additional RB1 Products

Product Documents for RB1 Antibody (1F8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for RB1 Antibody (1F8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle

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Protocols

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FAQs

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