RBM22 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-20104
![Western Blot: RBM22 Antibody [NBP2-20104]](https://resources.rndsystems.com/images/products/RBM22-Antibody-Western-Blot-NBP2-20104-img0001.jpg "Western Blot: RBM22 Antibody [NBP2-20104]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Chicken (97%), Mouse (100%), Rat (100%), Rhesus Macaque (100%), Xenopus (94%), Zebrafish (91%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human RBM22. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (94%).
Localization
Nucleus; Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RBM22 Antibody - BSA Free
Western Blot: RBM22 Antibody [NBP2-20104]
Western Blot: RBM22 Antibody [NBP2-20104] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: RBM22 Antibody [NBP2-20104] -
RBM22 antibody detects RBM22 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RBM22 protein stained by RBM22 antibody (NBP2-20104) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.
Applications for RBM22 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RBM22
Alternate Names
Cwc2, FLJ10290, fSAP47, functional spliceosome-associated protein 47, pre-mRNA-splicing factor RBM22, RNA binding motif protein 22, ZC3H16RNA-binding motif protein 22, Zinc finger CCCH domain-containing protein 16
Gene Symbol
RBM22
UniProt
Additional RBM22 Products
Product Documents for RBM22 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RBM22 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Customer Reviews for RBM22 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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