RCC2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32602

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RCC2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human RCC2. The exact sequence is proprietary.

Localization

Nucleus, nucleolus, Centromere, Spindle

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RCC2 Antibody - BSA Free

Western Blot: RCC2 Antibody [NBP1-32602]

Western Blot: RCC2 Antibody [NBP1-32602]

Western Blot: RCC2 Antibody [NBP1-32602] - KYSE-30 and KYSE-450 human esophageal cancer cells were transfected with a RCC2 over-expression plasmid or mock and RCC2 expression was detected by Western Blot. WB image submitted by a verified customer review.
Immunohistochemistry-Paraffin: RCC2 Antibody [NBP1-32602]

Immunohistochemistry-Paraffin: RCC2 Antibody [NBP1-32602]

Immunohistochemistry-Paraffin: RCC2 Antibody [NBP1-32602] - SW480 xenograft, using RCC2 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 mi.
Western Blot: RCC2 Antibody [NBP1-32602]

Western Blot: RCC2 Antibody [NBP1-32602]

Western Blot: RCC2 Antibody [NBP1-32602] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: RCC2 Antibody [NBP1-32602]

Western Blot: RCC2 Antibody [NBP1-32602]

Western Blot: RCC2 Antibody [NBP1-32602] - Sample(30 ug whole cell lysate)A:H1299B:MOLT4 7. 5% SDS PAGE, antibody diluted at 1:1000.

Applications for RCC2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:10 - 1:500

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 4 using NBP1-32602 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RCC2

RCC2 (regulator of chromosome condensation 2) is a member of the RCC1 family of guanine nucleotide exchange factors. RCC2 has been identified as part of the chromosomal passenger complex (CPC) which coordinates the chromosomal and cytoskeletal events of mitosis. Chromosomal passengers are proteins present in complexes with aurora B, inner centromeric protein (INCENP), survivin, and borealin. RCC2 has been demonstrated to be required for the recruitment of survivin and aurora B and is essential for proper chromosome segregation and the completion of cell cleavage. Loss of RCC2 inhibits spindle assembly, activates the spindle assembly checkpoint, and blocks cells in prometaphase.

Alternate Names

DKFZp762N0610, KIAA1470, protein RCC2, RCC1-like protein TD-60, regulator of chromosome condensation 2, TD60, TD-60, Telophase disk protein of 60 kDa

Gene Symbol

RCC2

Additional RCC2 Products

Product Documents for RCC2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for RCC2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Customer Reviews for RCC2 Antibody - BSA Free (1)

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Customer Images

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  • RCC2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: human esophageal cancer cells
    Species: Human
    Verified Customer | Posted 03/20/2020
    KYSE-30 and KYSE-450 esophageal cancer cells were transfected with a RCC2 overexpressing plasmid or mock and RCC2 expression was detected by Western Blot.
    RCC2 Antibody - BSA Free NBP1-32602

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Protocols

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FAQs

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