Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF

Name: Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF
Brand: R&D Systems
SKU: 10504-DC
Price: 454 USD
Availability: InStock
Rating: 5 (1 reviews)

R&D Systems | Catalog # 10504-DC

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems CHO-derived Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein (10504-DC)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

XP_015296329.1

Applications

Bioactivity

View all DC-SIGN/CD209 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey DC-SIGN/CD209 protein

Hemagglutinin Tag (YPYDVPDYA)	Cynomolgus Monkey DC-SIGN/CD209 (Lys62-Glu406) Accession # XP_015296329.1
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr of HA tag

Predicted Molecular Mass

40 kDa

SDS-PAGE

44-54 kDa, under reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells.
The ED₅₀ for this effect is 1.25-8 μg/mL.

Reviewed Applications

Read 1 review rated 5 using 10504-DC in the following applications:

Analytical SEC. (1 Review)

Scientific Data Images for Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF

Recombinant Cynomolgus Monkey DC-SIGN/CD209 HA-tag Protein Bioactivity.

Recombinant Cynomolgus Monkey DC-SIGN/CD209 HA-tag (Catalog # 10504-DC) supports the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. The ED₅₀ for this effect is 1.25-8 µg/mL.

Recombinant Cynomolgus Monkey DC-SIGN/CD209 HA-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey DC-SIGN/CD209 HA-tag (10504-DC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 44-54 kDa.

Formulation, Preparation, and Storage

10504-DC

Formulation	Supplied as a 0.2 μm filtered solution in PBS.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Background: DC-SIGN/CD209

Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family (1). The canonical DC-SIGN/CD209 isoform is a 40 kDa, 344 amino acid (aa) type II transmembrane protein, with the extracellular domain (ECD) containing a Ca2+-dependent carbohydrate-binding lectin domain (2). Within the ECD, cynomolgus DC-SIGN/CD209 shares 85% sequence identity with human DC-SIGN/CD209. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self-glycoproteins including ICAMs (2, 3). DC-SIGN/CD209 binds to butyrophilin 2A1 and this interaction can be blocked by HIV pp120. DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (4,5).