Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF

Catalog # Availability Size / Price Qty
10504-DC-050
Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF Data
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Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. The ED50 for this effect is 1.25-8 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey DC-SIGN/CD209 protein

Hemagglutinin Tag
(YPYDVPDYA)

Cynomolgus Monkey DC-SIGN/CD209
(Lys62-Glu406)
Accession # XP_015296329.1
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Tyr of HA tag
Predicted Molecular Mass
40 kDa
SDS-PAGE
44-54 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10504-DC

Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Data Images

Bioactivity View Larger

Recombinant Cynomolgus Monkey DC-SIGN/CD209 HA-tag (Catalog # 10504-DC) supports the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. The ED50 for this effect is 1.25-8 µg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey DC-SIGN/CD209 HA-tag (10504-DC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 44-54 kDa.

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Background: DC-SIGN/CD209

Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family (1). The canonical DC-SIGN/CD209 isoform is a 40 kDa, 344 amino acid (aa) type II transmembrane protein, with the extracellular domain (ECD) containing a Ca2+-dependent carbohydrate-binding lectin domain (2). Within the ECD, cynomolgus DC-SIGN/CD209 shares 85% sequence identity with human DC-SIGN/CD209. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self-glycoproteins including ICAMs (2, 3). DC-SIGN/CD209 binds to butyrophilin 2A1 and this interaction can be blocked by HIV pp120. DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (4,5).

References
  1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
  2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
  3. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
  4. Geijtenbeek, T.B. et al. (2000) Cell 100:575.
  5. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.
Long Name
Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1
Entrez Gene IDs
30835 (Human); 170786 (Mouse); 102121984 (Cynomolgus Monkey)
Alternate Names
CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin; CD209 molecule; CD209; CDSIGNHIV gpl20-binding protein; CLEC4L; CLEC4LC-type lectin domain family 4 member L; DCSIGN; DC-SIGN; DC-SIGN1; DC-SIGN1C-type lectin domain family 4, member L; DC-SIGNMGC129965; Dendritic cell-specific ICAM-3-grabbing non-integrin 1

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