Recombinant Cynomolgus Lgr4/GPR48 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10112-GP-050
Recombinant Cynomolgus Lgr4/GPR48 Fc Chimera Protein, CF Binding Activity
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Recombinant Cynomolgus Lgr4/GPR48 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human R-Spondin 3 Recombinant Human R‑Spondin 3 (Catalog # 3500-RS/CF) is coated at 0.5 μg/mL, 100 μL/well, Recombinant Cynomolgus Monkey Lgr4/GPR48 Fc Chimera binds with an ED50 of 6-36 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Lgr4/GPR48 protein
Cynomolgus Monkey Lgr4
(Ala25-Thr544)
Accession # NP_001252594
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Ala25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
84 kDa
SDS-PAGE
100-111 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10112-GP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human R-Spondin 3 Recombinant Human R‑Spondin 3 (Catalog # 3500-RS/CF) iscoated at 0.5 μg/mL, 100 μL/well, Recombinant Cynomolgus Monkey Lgr4/GPR48Fc Chimera (Catalog # 10112-GP) binds with an ED50 of 6-36 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey Lgr4/GPR48 Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 100-111 kDa and 200-220 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lgr4/GPR48

Lgr4 (leucine‑rich repeat GPR 4), also called GPR48 (G‑protein‑coupled receptor 48), is a seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors (1, 2). While this family includes receptors for hormones such as LH, FSH, TSH, and HCG, the subfamily comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling (1‑6). Lgr4 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors (5). It acts at least in part by enhancing Wnt‑dependent LRP phosphorylation, internalization of LRPs, and accumulation of beta ‑catenin (3, 4). Cynomolgus Monkey Lgr4 cDNA encodes 951 amino acids (aa), including a long N‑terminal extracellular domain with multiple LRR domains that may mediate ligand interaction. The LRR‑containing ECD of Cynomolgus Monkey Lgr4 shares 99% aa sequence identity with human Lgr4. Lgr4 is widely expressed in both embryo and adult. Expression of Lgr4 mRNA in adult humans is highest in pancreas, followed by liver, heart, muscle, brain, and placenta (1). In rodents, embryonic and adult expression includes liver, kidney, adrenals, bone/cartilage, and heart (2, 7‑9). Lgr4 deletion in the mouse affects development in areas of expression, for example, inhibiting fetal liver definitive erythropoiesis (9). Deletion of Lgr4 specifically from stem and progenitor cells in intestinal crypts induces loss of crypts due to insufficient Wnt signaling (5, 6). Lgr4 may be over‑expressed in carcinomas and may promote invasiveness and metastasis by down‑regulating p27Kip1 expression (10).

References
  1. Loh, E.D. et al. (2001) Biochem. Biophys. Res. Commun. 282:757.
  2. Hsu, S.Y. et al. (1998) Mol. Endocrinol. 12:1830.
  3. Carmon, K.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:11452.
  4. Glinka, A. et al. (2011) EMBO Rep. 12:1055.
  5. de Lau, W. et al. (2011) Nature 476:293.
  6. Ruffner, H. et al. (2012) PLoS ONE 7:e40975.
  7. Van Schoore, G. et al. (2005) Histochem. Cell Biol. 124:35.
  8. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:2241.
  9. Song, H. et al. (2008) J. Biol. Chem. 283:36687.
  10. Gao, Y. et al. (2006) Cancer Res. 66:11623.
Long Name
Leucine-rich Repeat Containing G Protein-coupled Receptor 4
Entrez Gene IDs
55366 (Human); 107515 (Mouse); 286994 (Rat); 102119503 (Cynomolgus Monkey)
Alternate Names
GPR48; GPR48G protein-coupled receptor 48; G-protein coupled receptor 48; leucine-rich repeat containing G protein-coupled receptor 4; Lgr4

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