Recombinant Cynomolgus Monkey CD200 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10086-CD-100
Recombinant Cynomolgus Monkey CD200 His-tag Protein, CF Binding Activity
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Recombinant Cynomolgus Monkey CD200 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD200 R1 Fc Chimera (Catalog # 3414-CD) is immobilized at 2 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey CD200 binds with an ED50 of 0.04-0.24 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey CD200 protein
Gln56-Gly257, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Gln56
Predicted Molecular Mass
23 kDa
SDS-PAGE
38-46 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10086-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human CD200 R1 Fc Chimera (Catalog # 3414-CD) is coated at 2 μg/mL, 100 μL/well, Recombinant Cynomolgus Monkey CD200 (Catalog # 10086-CD) binds with an ED50 of 0.04‑0.24 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey CD200 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 38-46 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD200

CD200, also known as OX-2, is a 45 kDa transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily (1). Mature cynomolgus CD200 consists of a 202 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain and one Ig-like C2-type domain, a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. Within the ECD, cynomolgus CD200 shares 95%, 78%, and 78% aa sequence identity with human, mouse, and rat CD200, respectively. CD200 is widely but not ubiquitously expressed (2). Its receptor (CD200 R1) is restricted primarily to mast cells, basophils, macrophages, and dendritic cells, which suggests myeloid cell regulation as the major function of CD200 (3-5). CD200 knockout mice are characterized by increased macrophage number and activation and are predisposed to autoimmune disorders (6). CD200 and CD200 R associate via their respective N-terminal Ig-like domains (7). In myeloid cells, CD200 R initiates inhibitory signals following receptor-ligand contact (4, 5, 8). In T cells, however, CD200 functions as a co-stimulatory molecule independent of the CD28 pathway (9). Several additional CD200 R-like molecules have been identified in human and mouse, but their capacity to interact with CD200 is controversial (10, 11). Several viruses encode CD200 homologs which are expressed on infected cells during the lytic phase (12, 13). Like CD200 itself, viral CD200 homologs also suppress myeloid cell activity, enabling increased viral propagation (3, 12-14).

References
  1. Vaine, C.A. and R.J. Soberman (2014) Adv. Immunol. 121:191.
  2. Wright, G.J. et al. (2001) Immunology 102:173.
  3. Shiratori, I. et al. (2005) J. Immunol. 175:4441.
  4. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
  5. Fallarino, F. et al. (2004) J. Immunol. 173:3748.
  6. Hoek, R.M. et al. (2000) Science 290:1768.
  7. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
  8. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
  9. Borriello, F. et al. (1997) J. Immunol. 158:4548.
  10. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
  11. Hatherley, D. et al. (2005) J. Immunol. 175:2469.
  12. Foster-Cuevas, M. et al. (2004) J. Virol. 78:7667.
  13. Cameron, C.M. et al. (2005) J. Virol. 79:6052.
  14. Langlais, C.L. et al. (2006) J. Virol. 80:3098.
Entrez Gene IDs
4345 (Human); 17470 (Mouse); 102146004 (Cynomolgus Monkey)
Alternate Names
antigen identified by monoclonal MRC OX-2; CD200 antigenMOX1; CD200 molecule; CD200; MOX1; MOX2; MOX2MRC; MRC OX-2 antigen; MRC; OX-2 membrane glycoprotein; OX-2

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