Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera, CF

R&D Systems | Catalog # 9660-CD

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera (9660-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

XP_005569274.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all CD40/TNFRSF5 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey CD40/TNFRSF5 protein
Cynomolgus Monkey CD40/TNFRSF5
(Glu21-Arg193)
Accession # XP_005569274.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu21

Predicted Molecular Mass

46 kDa

SDS-PAGE

57-64 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera is coated at 1 μg/mL, 100 μL/well, it binds to Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6420-CL) with an ED50 of 5-25 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera, CF

Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera Bioactivity

Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera Bioactivity

When Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera (Catalog # 9660-CD) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6420-CL) binds with an ED50 of 5-25 ng/mL.

Formulation, Preparation, and Storage

9660-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD40/TNFRSF5

CD40, also known as TNFRSF5, is a type I transmembrane glycoprotein member of the TNF receptor superfamily (1). Mature Cynomolgus Monkey CD40 consists of an extracellular domain, a transmembrane domain, and a cytoplasmic domain (2). The extracellular domain (21-193 aa) of Cynomolgus Monkey CD40 shares 96%, 58% and 56% amino acid (aa) sequence identity with human, mouse and rat CD40, respectively. CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells (4, 5). The extracellular domain has the cysteine-rich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to the aggregation of CD40 molecules resulting in the initiation of bidirectional intracellular signaling in both CD40 and CD40L expressing cells (6). CD40 ligation by CD40L promotes B cell activation and T cell‑dependent humoral responses (7, 8). CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy (9, 10). Dysregulation of CD40/CD40L expression and interactions contributes to the immune deficiency associated with HIV infection and AIDS (11, 12). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (13, 14).

References

  1. Banchereau, J. et al. (1994) Annu. Rev. Immunol. 12:881.
  2. Stamenkovic, I. et al. (1989) EMBO J. 8:1403.
  3. Eshel, D. et al. (2008) Mol. Immunol. 46:250.
  4. van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
  5. Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
  6. Eissner, G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
  7. Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
  8. Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
  9. Loskog, A.S. and A.G. Eliopoulos (2009) Semin. Immunol. 21:301.
  10. Hangalapura, B.N. et al. (2012) J. Gene Med. 14:416.
  11. Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373.
  12. Chougnet, C. (2003) J. Leukoc. Biol. 74:702.
  13. Pamukcu, B. et al. (2011) Ann. Med. 43:331.
  14. Hassan, G.S. et al. (2012) Immunobiology 217:521.

Alternate Names

CD40, TNFRSF5

Entrez Gene IDs

958 (Human); 21939 (Mouse); 171369 (Rat); 102118696 (Cynomolgus Monkey)

Gene Symbol

CD40

UniProt

XP_005569274.1

Additional CD40/TNFRSF5 Products

Product Documents for Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera, CF

For research use only

Citations for Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera, CF

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