Recombinant Cynomolgus Monkey IL-3R alpha Fc Protein, CF

Name: Recombinant Cynomolgus Monkey IL-3R alpha Fc Protein, CF
Brand: R&D Systems
SKU: 10597-R3
Price: 454 USD
Availability: InStock

R&D Systems | Catalog # 10597-R3

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems CHO-derived Recombinant Cynomolgus Monkey IL-3R alpha Fc Protein (10597-R3)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

EHH61867.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity

View all IL-3R alpha Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey IL-3R alpha protein

Cynomolgus Monkey IL-3R alpha (Arg18-Arg305) Accession # EHH61867.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg18

Predicted Molecular Mass

60 kDa

SDS-PAGE

85-100 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey IL-3R alpha Fc Chimera prootein (Catalog #10597-R3) is represent at 1 µg/mL (100 µg/mL), The concentration of Recombinant Human IL-3 Protein (Catalog # 203-IL) that produces 50% optimal binding response is found to be 8-40 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey IL-3R alpha Fc Protein, CF

Recombinant Cynomolgus Monkey IL-3R alpha Fc Chimera Protein Binding Activity.

When Recombinant Cynomolgus Monkey IL-3R alpha Fc Chimera (Catalog #10597-R3) is present at 1 µg/mL (100 µg/mL), the concentration of Recombinant Human IL-3 Protein (203-IL) that produces 50% optimal binding response is found to be 8-40 ng/mL.

Recombinant Cynomolgus Monkey IL-3R alpha Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey IL-3R alpha Fc Chimera (Catalog # 10597-R3) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-100 kDa and 170-200 kDa, respectively.

Formulation, Preparation, and Storage

10597-R3

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-3R alpha

IL-3 receptor chain alpha (IL‑3Ra) also known as CD123, is a member of the type 1 cytokine receptor family of proteins expressed mainly by activated T cells or mast cells (1-3). IL-3Ra is a 60-70 kDa receptor component that binds IL-3 with low affinity and forms one-half of the IL-3 receptor complex. IL-3Ra associates with the 130-140 kDa non-ligand binding IL-3Rb for high-affinity binding to IL-3 to form the heterodimeric receptor complex (4). IL-3Ra consists of an extracellular domain (ECD) with an N-terminal Ig-like C2-type domain and a cytokine-binding domain containing two fibronectin type-III domains with a WSXWS motif, a helical transmembrane segment and a cytoplasmic domain (3). Within the mature ECD, cynomolgus IL-3Ra shares 85% amino acid sequence identity with human IL-3Ra. An isoform lacking the N-terminal domain of ECD has been identified in mouse and human (5, 6). IL‑3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage‑committed cells (7). IL-3Ra is expressed on multiple cell types, including endothelial cells, monocytes, eosinophils, basophils plus mast cells, and plasmacytoid CD4+ T cells (8). IL-3Ra is over-expressed by Acute myeloid leukemia and this is associated with a poor prognosis in AML (9).

References

Broughton, S.E. et al. (2012) Immunol. Rev. 250:277.
Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
Broughton, S.E. et al. (2014) Acta Crystallogr F Struc Biol Commun. 70:358.
Broughton, S.E. et al. (2014) Cell Reports. 8:410.
Chen, J. et al. (2009) J. Biol. Chem. 284:5763.
Hamming, O.J. et al. (2012) J Biol Chem. 287:9454.
Ogorochi, T. and A. Miyajima (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, ed. Oxford University, New York p. 40.
Michalska, A. et al. (2020) Postepy Dermatol Alergol. 37:299.
Testa, U. et al. (2002) Blood 100:2980.