Recombinant Cynomolgus Monkey TIGIT Fc Chimera Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human CD155/PVR (Catalog # 2530-CD) is coated at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey TIGIT Fc Chimera that produces 50% optimal binding response is 12-72 ng/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived
    Cynomolgus Monkey TIGIT 
    (Met22-Pro142)
    Accession # XP_005548158
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Met22
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    40 kDa
  • SDS-PAGE
    43 - 60 kDa, reducing conditions
9380-TG
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Human CD155/PVR (Catalog # 2530-CD) is coated at 2.5 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey TIGIT Fc Chimera (Catalog # 9380-TG)binds with an ED50 of
12-72 ng/mL.
Background: TIGIT
TIGIT (T cell Immunoreceptor with Ig and ITIM domains), also called VSTM3 (V-set and transmembrane domain-containing 3), VSIG9 (V-set and Ig domain-containing 9) and WUCAM (Washington University cell adhesion molecule) is a 30-34 kDa type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins (1-4). Human TIGIT cDNA encodes 244 amino acids (aa) including a 21 aa signal sequence, a 120 aa extracellular region with a V-type Ig-like domain and two potential N-glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic domain with an ITIM motif (5). A 170 aa variant diverges after aa 166 (5). Within the ECD, human TIGIT shares only 68-75% aa sequence identity with mouse, porcine, canine, equine and bovine TIGIT. Cyno TIGIT shares 88.4% homology with human TIGIT. TIGIT is expressed on NK cells and subsets of activated, memory and regulatory T cells, and particularly on follicular helper T cells within secondary lymphoid organs (1, 2, 6-8). It binds to CD155/PVR/Necl-5 and Nectin-2/CD112/PVRL2 that appear on dendritic cells (DC) and endothelium (1-3, 7). Binding of TIGIT by DC induces IL-10 release and inhibits IL-12 production (2). Ligation of TIGIT on T cells down‑regulates TCR-mediated activation and subsequent proliferation, while NK cell TIGIT ligation blocks NK cell cytotoxicity (6-8). Through CD155 and Nectin-2, which also interact with DNAM-1/CD226 and CD96/Tactile, TIGIT is part of an interacting network of Ig superfamily members that may augment or oppose each other (3, 4, 6, 7). In particular, TIGIT binding to CD155 can antagonize the effects of DNAM-1 (6, 7). Soluble TIGIT is able to compete with DNAM-1 for CD155 binding and attenuates T cell responses, while mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges (2, 3, 8).
  • References:
    1. Boles, K.S. et al. (2009) Eur. J. Immunol. 39:695.
    2. Yu, X. et al. (2009) Nat. Immunol. 10:48.
    3. Levin, S.D. et al. (2011) Eur. J. Immunol. 41:902.
    4. Xu, Z. et al. (2010) Cell. Mol. Immunol. 7:11.
    5. SwissProt Accession # Q495A1.
    6. Seth, S. et al. (2009) Eur. J. Immunol. 39:3160.
    7. Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858.
    8. Joller, N. et al. (2011) J. Immunol. 83:1338.
  • Long Name:
    T Cell Immunoreceptor with Ig and ITIM Domains
  • Entrez Gene IDs:
    201633 (Human); 100043314 (Mouse); 363784 (Rat); 102121588 (Cynomolgus Monkey)
  • Alternate Names:
    TIGIT; VSIG9; VSTM3; WUCAM
Related Research Areas

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