>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human CD155/PVR (Catalog # 2530-CD)
is coated at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey TIGIT Fc Chimera that produces 50% optimal binding response is 12-72 ng/mL.
When Recombinant Human CD155/PVR (Catalog # 2530-CD) is coated at 2.5 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey TIGIT Fc Chimera (Catalog # 9380-TG)binds with an ED50 of 12-72 ng/mL.
TIGIT (T cell Immunoreceptor with Ig and ITIM domains), also
called VSTM3 (V-set and transmembrane domain-containing 3), VSIG9 (V-set and Ig domain-containing 9) and WUCAM (Washington University cell adhesion molecule)
is a 30-34 kDa type I transmembrane protein that is a member of the CD28 family
within the Ig superfamily of proteins (1-4). Human TIGIT cDNA encodes 244 amino
acids (aa) including a 21 aa signal sequence, a 120 aa extracellular region
with a V-type Ig-like domain and two potential N-glycosylation site, a 21 aa
transmembrane sequence, and an 82 aa cytoplasmic domain with an ITIM motif (5).
A 170 aa variant diverges after aa 166 (5). Within the ECD, human TIGIT shares
only 68-75% aa sequence identity with mouse, porcine, canine, equine and bovine
TIGIT. Cyno TIGIT shares 88.4% homology with human TIGIT. TIGIT is expressed on
NK cells and subsets of activated, memory and regulatory T cells, and
particularly on follicular helper T cells within secondary lymphoid organs (1, 2, 6-8). It binds to CD155/PVR/Necl-5 and Nectin-2/CD112/PVRL2 that appear on
dendritic cells (DC) and endothelium (1-3, 7). Binding of TIGIT by DC induces IL-10 release
and inhibits IL-12 production (2). Ligation of TIGIT on T cells down‑regulates
TCR-mediated activation and subsequent proliferation, while NK cell TIGIT
ligation blocks NK cell cytotoxicity (6-8). Through CD155 and Nectin-2, which
also interact with DNAM-1/CD226 and CD96/Tactile, TIGIT is part of an
interacting network of Ig superfamily members that may augment or oppose each
other (3, 4, 6, 7). In particular, TIGIT binding to CD155 can antagonize the
effects of DNAM-1 (6, 7). Soluble TIGIT is able to compete with DNAM-1 for
CD155 binding and attenuates T cell responses, while mice lacking TIGIT show
increased T cell responses and susceptibility to autoimmune challenges (2, 3, 8).
Boles, K.S. et al. (2009) Eur. J. Immunol. 39:695.
Yu, X. et al. (2009) Nat. Immunol. 10:48.
Levin, S.D. et al. (2011) Eur. J. Immunol. 41:902.
Xu, Z. et al. (2010) Cell. Mol. Immunol. 7:11.
SwissProt Accession # Q495A1.
Seth, S. et al. (2009) Eur. J. Immunol. 39:3160.
Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858.
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