Recombinant Cynomolgus Monkey TIM-3 Fc Chimera Protein, CF

  
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-3 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived
    Cynomolgus Monkey TIM-3
    (Ser22-Arg201)
    Accession # EHH54703
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser22 & Val24
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    46.3 kDa (monomer)
  • SDS-PAGE
    60-75 kDa, reducing conditions
7914-TM
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant CynomolgusMonkey TIM-3 (Catalog #
7914-TM) inhibits anti-CD3 antibody induced IL-2secretion in human T lymphocytes. The ED50 for this effect is0.5-3 μg/mL.
Background: TIM-3

TIM‑3 (T cell immunoglobulin and mucin domain‑3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig‑like V‑type domain and a Ser/Thr‑rich mucin stalk region (1, 2). Mature cynomolgus TIM‑3 consists of a 182 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail. Within the ECD, cynomolgus (or crab‑eating macaque) monkey TIM‑3 shares 81%, 57%, and 56% aa sequence identity with human, mouse, and rat TIM‑3, respectively. TIM‑3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg) (3‑10). Its binding to Galectin‑9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti‑tumor immunity (11). TIM‑3 ligation attenuates CD8+ and Th1 cell responses (11‑13) and promotes the activity of Treg and myeloid derived suppressor cells (8, 11, 13, 14). In addition, dendritic cell‑expressed TIM‑3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross‑presentation of apoptotic cell antigens (3). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (6). Soluble TIM‑3 is also reported to inhibit the response of T cells to both Ag‑induced and concurrent CD3/CD28 stimulation (15). By contrast, TIM‑3 interactions with Galectin‑9 can trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (10).

  • References:
    1. Sakuishi, K. et al. (2011) Trends Immunol. 32:345.
    2. Anderson, A.C. (2012) Curr. Opin. Immunol. 24:213.
    3. Nakayama, M. et al. (2009) Blood 113:3821.
    4. Anderson, A.C. et al. (2007) Science 318:1141.
    5. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
    6. Chiba, S. et al. (2012) Nat. Immunol. 13:832.
    7. Monney, L. et al. (2002) Nature 415:536.
    8. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
    9. Ndhlovu, L.C. et al. (2012) Blood 119:3734.
    10. Gleason, M.K. et al. (2012) Blood 119:3064.
    11. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
    12. Sakhdari, A. et al. (2012) PLoS ONE 7:e40146.
    13. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
    14. Dardalhon, V. et al. (2010) J. Immunol. 185:1383.
    15. Geng, H. et al. (2006) J. Immunol. 176:1411.
  • Long Name:
    T Cell Immunoglobulin Mucin-3
  • Entrez Gene IDs:
    84868 (Human); 171285 (Mouse); 102141722 (Cynomolgus Monkey)
  • Alternate Names:
    CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; TIM-3; TIMD-3; TIMD3KIM-3
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