Recombinant Cynomolgus Monkey TIM-3 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
7914-TM-050
Recombinant Cynomolgus Monkey TIM-3 Fc Chimera Protein, CF Bioactivity
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Recombinant Cynomolgus Monkey TIM-3 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-3 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived cynomolgus monkey TIM-3 protein
Cynomolgus Monkey TIM-3
(Ser22-Arg201)
Accession # EHH54703
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ser22 & Val24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
46.3 kDa (monomer)
SDS-PAGE
60-75 kDa, reducing conditions

Product Datasheets

7914-TM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Bioactivity Recombinant Cynomolgus Monkey TIM-3 Fc Chimera Protein, CF Bioactivity View Larger

Recombinant CynomolgusMonkey TIM-3 (Catalog #
7914-TM) inhibits anti-CD3 antibody induced IL-2secretion in human T lymphocytes. The ED50 for this effect is0.5-3 μg/mL.

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Background: TIM-3

TIM‑3 (T cell immunoglobulin and mucin domain‑3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig‑like V‑type domain and a Ser/Thr‑rich mucin stalk region (1, 2). Mature cynomolgus TIM‑3 consists of a 182 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail. Within the ECD, cynomolgus (or crab‑eating macaque) monkey TIM‑3 shares 81%, 57%, and 56% aa sequence identity with human, mouse, and rat TIM‑3, respectively. TIM‑3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg) (3‑10). Its binding to Galectin‑9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti‑tumor immunity (11). TIM‑3 ligation attenuates CD8+ and Th1 cell responses (11‑13) and promotes the activity of Treg and myeloid derived suppressor cells (8, 11, 13, 14). In addition, dendritic cell‑expressed TIM‑3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross‑presentation of apoptotic cell antigens (3). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (6). Soluble TIM‑3 is also reported to inhibit the response of T cells to both Ag‑induced and concurrent CD3/CD28 stimulation (15). By contrast, TIM‑3 interactions with Galectin‑9 can trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (10).

References
  1. Sakuishi, K. et al. (2011) Trends Immunol. 32:345.
  2. Anderson, A.C. (2012) Curr. Opin. Immunol. 24:213.
  3. Nakayama, M. et al. (2009) Blood 113:3821.
  4. Anderson, A.C. et al. (2007) Science 318:1141.
  5. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
  6. Chiba, S. et al. (2012) Nat. Immunol. 13:832.
  7. Monney, L. et al. (2002) Nature 415:536.
  8. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
  9. Ndhlovu, L.C. et al. (2012) Blood 119:3734.
  10. Gleason, M.K. et al. (2012) Blood 119:3064.
  11. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  12. Sakhdari, A. et al. (2012) PLoS ONE 7:e40146.
  13. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
  14. Dardalhon, V. et al. (2010) J. Immunol. 185:1383.
  15. Geng, H. et al. (2006) J. Immunol. 176:1411.
Long Name
T Cell Immunoglobulin Mucin-3
Entrez Gene IDs
84868 (Human); 171285 (Mouse); 102141722 (Cynomolgus Monkey)
Alternate Names
CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; TIM-3; TIMD-3; TIMD3KIM-3

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Primary Antibodies

Human IgG1 Antibody

Recombinant Proteins

Reconstitution Buffers

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Recombinant Cynomolgus Monkey TIM-3 Fc Chimera Protein, CF
By Anonymous on 03/24/2018
Application: Binding assay/Protein-protein interaction