Recombinant Cynomolgus/Rhesus Macaque IL-17F His Protein, CF Summary
Arg31-Gln163, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Cynomolgus Monkey/Rhesus Macaque IL-17F (Catalog # 10225-IL) induces IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50for this effect is 5-40 ng/mL.
2 μg/lane of Recombinant Cynomolgus/Rhesus Macaque IL-17F His-tag (Catalog # 10225-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 17-25 kDa and 30-50 kDa, respectively.
The Interleukin 17 (IL-17) protein family, composed of six members (IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N‑terminus. With the exception of IL-17B which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Cynomolgus IL-17F cDNA encodes a 163 amino acid (aa) protein with a putative 30 aa signal peptide. Mature cynomolgus IL-17F shares 96% and 100% aa sequence identity with human and rhesus macaque IL-17F, respectively. IL-17F is expressed in activated CD4+ T-cells and activated monocytes. Five receptors (IL-17 RA-RE) have been identified. Although the ligands for IL-17 RD and RE are not known yet, it is reported that IL-17 RA binds IL-17A, and IL-17 RB binds IL-17B and IL-17E. IL-17 RC binds IL‑17A and IL‑17F with similarly high affinity and functions as a receptor for both IL-17A and IL‑17F (3, 4). The biological activities mediated by IL-17F are similar to those of IL-17A. IL-17F stimulates production of IL-6, IL-8, G-CSF, and regulates cartilage matrix turnover by increasing matrix release and inhibiting new matrix synthesis (5). IL-17F also inhibits angiogenesis and induces production of IL-2, TGF-beta, and monocyte chemoattractant protein-1 in endothelial cells (6).
- Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
- Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
- Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
- Shen, F. & S. L. Gaffen (2008) Cytokine 41:92.
- Hot, A. and Miossec, P. (2011) Ann. Rheum. Dis. 70:727.
- Starnes, T. et al. (2001) J. Immunol. 167:4137.
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