Recombinant Human ACLP His-tag Protein, CF

Catalog # Availability Size / Price Qty
6425-AC-050
Recombinant Human ACLP His-tag Protein, CF Binding Activity
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Recombinant Human ACLP His-tag Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human LRP-6 Fc Chimera (Catalog # 1505-LR) is coated at 2 ug/mL, 100 uL/well, Recombinant ACLP binds with an ED50 of 1-8 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human ACLP protein
Gln26-Phe1158, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained. Gln26 inferred from enzymatic pyroglutamate treatment revealing Thr27.
Predicted Molecular Mass
130 kDa
SDS-PAGE
160-180 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6425-AC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human LRP-6 Fc Chimera (Catalog # 1505-LR) is coated at 2 μg/mL, 100 μL/well, Recombinant Human ACLP (Catalog # 6425-AC) binds with an ED50 of 1‑8 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human ACLP His-tag (Catalog# 6425-AC) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 160-180  kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ACLP

Aortic carboxypeptidase-like protein (ACLP), also known as adipocyte enhancer binding protein (AEBP1) (1, 2), is a secreted ECM-associated protein expressed primarily by perivascular and vascular cells and is up-regulated in activated vascular cells following vascular injury (3). Pro-ACLP is composed of an N-terminal signal sequence that is cleaved off in the mature form, a Lys/Pro rich motif (aa 47-326), a Glu rich motif (aa 1079-1136), a collagen binding discoidin-like domain (aa 383-540) and a peptidase-like domain (aa 560-942) (4, 5). Mature human ACLP shares 84% aa sequence identity with mouse and rat ACLP. In the liver, ACLP is specifically expressed in hepatic stellate cells (HSCs). Although unrelated to WNT proteins, ACLP can specifically bind to Frizzled-8 and Lrp6 to form a ternary complex to activate the canonical WNT pathway and exacerbates nonalcoholic steatohepatitis (NASH) pathology, indicating that NASH can be treated by targeting ACLP (6). Diseases associated with ACLP-1 include Ehlers-Danlos syndrome classic-like 2 (EDSCLL2) (7) and gastroschisis. Over-expression of ACLP-1 is associated with glioblastoma (8).

References
  1. He, G.P. et al.(1995) Nature. 378: 92.
  2. Ro, H.S. et al. (2001) Gene. 280:123.
  3. Layne, M.D. et al. (2002) Cir. Res. 90:728.
  4. Tumelty, K.E. et al. (2014) J. Biol. Chem. 289:2526.
  5. Layne, M.D. et al. (1998) J. Biol. Chem. 273:15654.
  6. Teratani, T. et al. (2018) J Clin Invest. 128:1581.
  7. Blackburn, P.R. et al. (2018) Am J Hum Genet. 102:696.
  8. Ladha, J. et al. (2012) Mol Cancer Res. 10:1039.
Long Name
Aortic Carboxypeptidase-like Protein
Entrez Gene IDs
165 (Human)
Alternate Names
ACLP; ACLPadipocyte enhancer binding protein 1; AE binding protein 1; AE-binding protein 1adipocyte enhancer-binding protein 1; AEBP1; Aortic carboxypeptidase-like protein; FLJ33612

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