Recombinant Human ACLP His-tag Protein, CF Summary
Gln26-Phe1158, with a C-terminal 10-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human LRP-6 Fc Chimera (Catalog # 1505-LR) is coated at 2 μg/mL, 100 μL/well, Recombinant Human ACLP (Catalog # 6425-AC) binds with an ED50 of 1‑8 μg/mL.
2 μg/lane of Recombinant Human ACLP His-tag (Catalog# 6425-AC) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 160-180 kDa.
Aortic carboxypeptidase-like protein (ACLP), also known as adipocyte enhancer binding protein (AEBP1) (1, 2), is a secreted ECM-associated protein expressed primarily by perivascular and vascular cells and is up-regulated in activated vascular cells following vascular injury (3). Pro-ACLP is composed of an N-terminal signal sequence that is cleaved off in the mature form, a Lys/Pro rich motif (aa 47-326), a Glu rich motif (aa 1079-1136), a collagen binding discoidin-like domain (aa 383-540) and a peptidase-like domain (aa 560-942) (4, 5). Mature human ACLP shares 84% aa sequence identity with mouse and rat ACLP. In the liver, ACLP is specifically expressed in hepatic stellate cells (HSCs). Although unrelated to WNT proteins, ACLP can specifically bind to Frizzled-8 and Lrp6 to form a ternary complex to activate the canonical WNT pathway and exacerbates nonalcoholic steatohepatitis (NASH) pathology, indicating that NASH can be treated by targeting ACLP (6). Diseases associated with ACLP-1 include Ehlers-Danlos syndrome classic-like 2 (EDSCLL2) (7) and gastroschisis. Over-expression of ACLP-1 is associated with glioblastoma (8).
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- Ro, H.S. et al. (2001) Gene. 280:123.
- Layne, M.D. et al. (2002) Cir. Res. 90:728.
- Tumelty, K.E. et al. (2014) J. Biol. Chem. 289:2526.
- Layne, M.D. et al. (1998) J. Biol. Chem. 273:15654.
- Teratani, T. et al. (2018) J Clin Invest. 128:1581.
- Blackburn, P.R. et al. (2018) Am J Hum Genet. 102:696.
- Ladha, J. et al. (2012) Mol Cancer Res. 10:1039.
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