Recombinant Human LRP-6 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1505-LR-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (5)
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Recombinant Human LRP-6 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. rhLRP-6/Fc Chimera binds rhDkk-1, biotin with an apparent KD <25 nM. Biotinylated rmWnt-3a immobilized on a streptavidin-coated plate at 1 µg/mL can bind rhLRP-6/Fc Chimera with a linear range of 0.2-10 µg/mL.
Optimal concentrations should be determined by each laboratory for each application.

Source
Mouse myeloma cell line, NS0-derived human LRP-6 protein
Human LRP-6
(Ala20 - Pro1368)
Accession # O75581
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ala20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
180 kDa (monomer)
SDS-PAGE
200 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1505-LR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: LRP-6

The low-density lipoprotein (LDL) receptor-related protein 5 (LRP5) and LRP6 constitute a distinct subgroup of the LDL receptor family. Both LRP5 and LRP6 are type I transmembrane proteins that function as co-receptors with Frizzled (FZD) in the canonical Wnt signaling pathway (1, 2). LRP6 cDNA encodes a 1613 amino acid residue (aa) precursor with a 19 aa signal sequence, a 1353 aa extracellular region, a 23 aa transmembrane (TM) segment and a 218 aa cytoplasmic domain (3). The extracellular region contains four N-terminal cysteine-rich EGF-like repeats, followed by three cysteine-rich LDLR repeats. This pattern of the EGF and LDLR repeat arrangement is different than that typically found in other LDL receptor family proteins. The intracellular region of LRP6 contains protein-protein interaction motifs and is required for canonical Wnt signal transduction (4). Human LRP-6 shares 98% and 74% aa sequence identity with mouse LRP-6 and human LRP-5, respectively. Based on the current model, canonical Wnt signaling requires the interaction of Wnt with FZD and LRP to form a trimeric complex which signals downstream to stabilize cytoplasmic  beta -catenin. The stabilized beta -catenin is then translocated to the nucleus where it complexes with the transcription factor LEF/TCF to regulate the transcription of target genes (5). LRP6 has also been shown to interact with the Dickkopf proteins (DKKs), which are modulators of Wnt signaling (6 - 8). Binding of DKK-1 to LRP6 dissociates LRP6 from FZD, and antagonizes the formation of the functional receptor complex. On cells where the transmembrane proteins Kremens are also present, a ternary complex of LRP6, DKK-1 and Kremen is formed to trigger the internalization of the complex and removal LRP6 from the cell surface. Thus, DKK-1 and Kremen function synergistically to antagonize LRP6-mediated Wnt activity. Although DKK-2 also functions as a Wnt antagonist on cells that express Kremen, DKK-2 binding to LRP-6 in the absence of Kremen activates rather than inhibits LRP mediated beta -catenin signaling (9, 10).

References
  1. Howell, B.W. and J. Herz (2001) Curr. Op. Neurobiology 11:74.
  2. Pinson, K.I. et al. (2000) Nature 407:535.
  3. Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248:879.
  4. Tamai, K. et al. (2000) Nature 407:530.
  5. Schweizer, L. and H. Varmus (2003) BMC Cell Biology 4:4.
  6. Mao, B. et al. (2001) Nature 411:321.
  7. Semenov, M.V. et al. (2001) Curr. Biol. 11:951.
  8. Bafico, A. et al. (2001) Nature Cell Biol. 3:683.
  9. Zorn, A.M. (2001) Curr. Biol. 11:R592.
  10. Mao, B. et al. (2002) Nature 417:664.
Long Name
LDL Receptor-related Protein 6
Entrez Gene IDs
4040 (Human); 16974 (Mouse)
Alternate Names
ADCAD2; FLJ90062; FLJ90421; low density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LRP6; LRP-6

Citations for Recombinant Human LRP-6 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Novel Bi-specific Domain Antibody to LRP6 Inhibits Wnt and R-spondin Ligand-induced Wnt Signaling and Tumor Growth
    Mol Cancer Res, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Affinity Purification
  2. Thiazolidinediones increase the wingless-type MMTV integration site family (WNT) inhibitor Dickkopf-1 in adipocytes: a link with osteogenesis.
    Authors: Gustafson B, Eliasson B, Smith U
    Diabetologia, 2010;53(3):536-40.
    Species: Human
    Sample Types: Serum
    Applications: ELISA (Capture)
  3. Cooperative folding and ligand-binding properties of LRP6 beta-propeller domains.
    Authors: Liu CC, Pearson C, Bu G
    J. Biol. Chem., 2009;284(22):15299-307.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  4. Nephroblastoma overexpressed (Nov) inhibits osteoblastogenesis and causes osteopenia.
    Authors: Rydziel S, Stadmeyer L, Zanotti S, Durant D, Smerdel-Ramoya A, Canalis E
    J. Biol. Chem., 2007;282(27):19762-72.
    Species: Mouse
    Sample Types: Protein
    Applications: Bioassay
  5. Dickkopf-1 is a master regulator of joint remodeling.
    Authors: Diarra D, Stolina M, Polzer K, Zwerina J, Ominsky MS, Dwyer D, Korb A, Smolen J, Hoffmann M, Scheinecker C, van der Heide D, Landewe R, Lacey D, Richards WG, Schett G
    Nat. Med., 2007;13(2):156-63.
    Species: Human
    Sample Types: Serum
    Applications: ELISA (Capture)

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