Recombinant Human ALCAM/CD166 Fc Chimera Avi-tag Protein, CF
Recombinant Human ALCAM/CD166 Fc Chimera Avi-tag Protein, CF Summary
Accession # AAB59499.1
Complete Your Research
Complete Your Experiment
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 250 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human CD6 Fc Chimera (627-CD) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ALCAM/CD166 Fc Chimera Avi-tag Protein (Catalog # AVI7187) binds with an ED50 of 1.50-12.0 ng/mL.
2 μg/lane of Recombinant Human ALCAM/CD166 Fc Chimera Avi-tag Protein (Catalog # AVI7187) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 100-120 kDa and 200-240 kDa, respectively.
Activated leukocyte cell adhesion molecule (ALCAM), also known as CD166, is a surface glycoprotein belonging to the immunoglobulin superfamily (1, 2). ALCAM, along with MCAM and BCAM/Lu, form a small subgroup of adhesion molecules involved in tissue development and maintenance, neurogenesis, and regulation of immune responses (3). Mature human ALCAM consists of an extracellular domain (ECD) containing 2 IgV and 3 IgC domains, a transmembrane domain, and a short cytoplasmic domain (1, 2). Within the ECD, human ALCAM shares 93% amino acid sequence identity with both mouse and rat ALCAM. An isoform of ALCAM with a shorter stalk region in the ECD is known to exist and is associated with higher shedding tendency and decreased cell adhesion (2). Initially found expressed on activated leukocytes, ALCAM expression has been detected in a wide variety of tissues and cells (3, 4). In addition to cell adhesion, other ALCAM functions include leukocyte migration across the blood brain barrier, T-cell activation, and osteogenesis, (2, 5, 6). Overexpression of ALCAM is often associated with poor prognosis in various human tumors, such as bladder cancer, prostate cancer, melanoma, and liver cancer (2, 7). Our Avi-tag Biotinylated Recombinant Human ALCAM features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.
- Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
- Ferragut, F. et al. (2021) Cytokine Growth Factor Rev. 61:27.
- Swart, G.W. (2002) Eur J Cell Biol. 81:313).
- Zimmerman, A.W. et al. (2006) Blood 107:3212.
- Masedunskas, A. et al. (2006) FEBS Lett. 580:2637.
- Cayrol, R. et al. (2008) Nat. Immunol. 9:137.
- Darvishi, B. et al. (2020) Exp. & Mol. Path 115:104443.
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Human ALCAM/CD166 Fc Chimera Avi-tag Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human ALCAM/CD166 Fc Chimera Avi-tag Protein, CF and earn rewards!
Have you used Recombinant Human ALCAM/CD166 Fc Chimera Avi-tag Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image