Biotinylated proteins can be powerful tools to assess protein/protein interactions in a range of assay formats, including immunoprecipitation, flow cytometry, immunoassays, and surface plasmon resonance. To be successful, it is crucial that biotinylation does not affect protein activity. For this reason, we hold our biotinylated proteins to the same specifications as their unlabeled counterparts.
Features and benefits
New Avi-Tag proteins allow for more control over the extent and consistency of biotinylation.
Labeling via carbohydrates is less likely to affect binding activity
Traditional labeling via amines can produce enhanced sensitivity
All proteins are tested in the same bioassay as unlabeled proteins to ensure specificity
Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) is immobilized at 2 µg/mL, Biotinylated Recombinant Human PD-L1 Avi-tag protein (Catalog # AVI9049) binds with an ED50 in the low µg/mL range (Left Panel). Recombinant Human IL-2 R alpha Fc Chimera (Catalog # 1020-RL) is coated at 1 µg/mL, Recombinant, Biotinylated Human IL-2 (Catalog # BT202) binds with an ED50 of in the low ng/mL (Middle panel). When Recombinant Human VSIG3 Fc Chimera (Catalog # 9229-VS) is coated at 5 µg/mL, Biotinylated Recombinant Human VISTA/B7-H5 Fc Chimera (Catalog # BT7126) binds in the low µg/mL range (Right panel).
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Our Custom Protein Development Team is comprised of experts dedicated to providing proteins that meet your specifications. This includes developing proteins from scratch, manufacturing to animal-free/GMP specifications, or adding labels such as biotin.