Recombinant Human ALCAM Fc Chimera (HEK293-expressed), CF
Recombinant Human ALCAM Fc Chimera (HEK293-expressed), CF Summary
Optimal dilutions should be determined by each laboratory for each application.
Accession # AAB59499
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
ALCAM (activated leukocyte cell adhesion molecule), designated CD166 and also called MEMD and SC‑1/DM‑GRASP/BEN in the chicken, is a 100‑110 kDa type I transmembrane glycoprotein and a member of the Ig CAM family within the immunoglobulin superfamily (1). ALCAM is expressed on thymic epithelium, microvascular endothelium, activated lymphocytes and monocytes, and monocyte-derived dendritic cells (1, 2). Human ALCAM cDNA encodes 583 amino acid (aa), including signal peptide (27 aa), extracellular domain (ECD, 500 aa) with two V‑type and three C2‑type Ig-like domains, transmembrane (22 aa) and cytoplasmic (34 aa) domains (1). Human ALCAM ECD shares 93%, 95%, and 96% aa sequence identity with mouse/rat, bovine and porcine/equine ALCAM, respectively. A 570 aa isoform lacks aa 503‑515, while a 555 aa form lacks most of the cytoplasmic domain. A secreted isoform in endothelial cells that is truncated at aa 133 (sALCAM) antagonizes full‑length ALCAM (3, 4). ALCAM mediates low-affinity adhesion with itself or the cysteine-rich scavenger receptor CD6 to regulate T cell development, immunological synapses (IS), and cell migration through endothelial junctions (1‑11). ALCAM on thymic epithelia mediates adhesion to CD6 on CD4+CD8+ T cells (6). Adhesion of ALCAM-expressing antigen presenting cells and CD6‑expressing T cells stabilizes the early IS, while later it enhances CD3 effects on T cell proliferation, CD25 expression, and Th1 commitment (2, 7, 8). High ALCAM expression at the blood‑brain barrier in active multiple sclerosis, and its mouse model (EAE), promotes leukocyte migration to the brain (8, 9). High ALCAM expression on melanoma cell lines appears to be pro-metastatic, but anti-metastatic activity has been reported in breast cancer (3, 10, 11). ALCAM may influence expression or adhesion of the neuronal adhesion molecule NCAM-L1, both in the developing retina and invasive melanoma (3, 12).
- Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
- Zimmerman, A.W. et al. (2006) Blood 107:3212.
- van Kilsdonk, J.W.J. et al. (2008) Cancer Res. 68:3671.
- Ikeda, K. and T. Quertermous (2004) J. Biol. Chem. 279:55315.
- van Kempen, L.C. et al. (2001) J. Biol. Chem. 276:25783.
- Castro, M.A.A. et al. (2007) J. Immunol. 178:4351.
- Nair, P. et al. (2010) Clin. Exp. Immunol. 162:116.
- Masedunskas, A. et al. (2006) FEBS Lett. 580:2637.
- Cayrol, R. et al. (2008) Nat. Immunol. 9:137.
- Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
- King, J.A. et al. (2010) Mol. Cancer 9:266.
- Buhusi, M. et al. (2009) J. Neurosci. 29:15630.
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Human ALCAM Fc Chimera (HEK293-expressed), CF
Average Rating: 3 (Based on 1 Review)
Have you used Recombinant Human ALCAM Fc Chimera (HEK293-expressed), CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image