Recombinant Human ALCAM Fc Chimera (HEK293-expressed), CF

Catalog # Availability Size / Price Qty
7187-AL-100
Product Details
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Recombinant Human ALCAM Fc Chimera (HEK293-expressed), CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to block adhesion of HuT 78 human cutaneous T cell lymphoma cells to immobilized Recombinant Human CD6 Fc Chimera (Catalog # 627-CD). The ED50 for this effect is 0.1‑0.5 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Human embryonic kidney cell, HEK293-derived human ALCAM/CD166 protein
Human ALCAM/CD166
(Met1-Ala526)
Accession # AAB59499
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Trp28
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
82.7 kDa (monomer)
SDS-PAGE
85-120 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7187-AL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ALCAM/CD166

ALCAM (activated leukocyte cell adhesion molecule), designated CD166 and also called MEMD and SC‑1/DM‑GRASP/BEN in the chicken, is a 100‑110 kDa type I transmembrane glycoprotein and a member of the Ig CAM family within the immunoglobulin superfamily (1). ALCAM is expressed on thymic epithelium, microvascular endothelium, activated lymphocytes and monocytes, and monocyte-derived dendritic cells (1, 2). Human ALCAM cDNA encodes 583 amino acid (aa), including signal peptide (27 aa), extracellular domain (ECD, 500 aa) with two V‑type and three C2‑type Ig-like domains, transmembrane (22 aa) and cytoplasmic (34 aa) domains (1). Human ALCAM ECD shares 93%, 95%, and 96% aa sequence identity with mouse/rat, bovine and porcine/equine ALCAM, respectively. A 570 aa isoform lacks aa 503‑515, while a 555 aa form lacks most of the cytoplasmic domain. A secreted isoform in endothelial cells that is truncated at aa 133 (sALCAM) antagonizes full‑length ALCAM (3, 4). ALCAM mediates low-affinity adhesion with itself or the cysteine-rich scavenger receptor CD6 to regulate T cell development, immunological synapses (IS), and cell migration through endothelial junctions (1‑11). ALCAM on thymic epithelia mediates adhesion to CD6 on CD4+CD8+ T cells (6). Adhesion of ALCAM-expressing antigen presenting cells and CD6‑expressing T cells stabilizes the early IS, while later it enhances CD3 effects on T cell proliferation, CD25 expression, and Th1 commitment (2, 7, 8). High ALCAM expression at the blood‑brain barrier in active multiple sclerosis, and its mouse model (EAE), promotes leukocyte migration to the brain (8, 9). High ALCAM expression on melanoma cell lines appears to be pro-metastatic, but anti-metastatic activity has been reported in breast cancer (3, 10, 11). ALCAM may influence expression or adhesion of the neuronal adhesion molecule NCAM-L1, both in the developing retina and invasive melanoma (3, 12).

References
  1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
  2. Zimmerman, A.W. et al. (2006) Blood 107:3212.
  3. van Kilsdonk, J.W.J. et al. (2008) Cancer Res. 68:3671.
  4. Ikeda, K. and T. Quertermous (2004) J. Biol. Chem. 279:55315.
  5. van Kempen, L.C. et al. (2001) J. Biol. Chem. 276:25783.
  6. Castro, M.A.A. et al. (2007) J. Immunol. 178:4351.
  7. Nair, P. et al. (2010) Clin. Exp. Immunol. 162:116.
  8. Masedunskas, A. et al. (2006) FEBS Lett. 580:2637.
  9. Cayrol, R. et al. (2008) Nat. Immunol. 9:137.
  10. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
  11. King, J.A. et al. (2010) Mol. Cancer 9:266.
  12. Buhusi, M. et al. (2009) J. Neurosci. 29:15630.
Long Name
Activated Leukocyte Cell Adhesion Molecule
Entrez Gene IDs
214 (Human); 11658 (Mouse); 101867493 (Cynomolgus Monkey)
Alternate Names
activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion moleculeFLJ38514; ALCAM; CD166 antigen; CD166; MEMDMGC71733

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Recombinant Human ALCAM Fc Chimera (HEK293-expressed), CF
By Anonymous on 08/04/2020
Application: Binding assay/Protein-protein interaction