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Recombinant Human B7-1/CD80 Fc Chimera Protein, CF

Name: Recombinant Human B7-1/CD80 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10133-B1
Price: 3120 USD
Availability: InStock

R&D Systems | Catalog # 10133-B1

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems CHO-derived Recombinant Human B7-1/CD80 Fc Chimera Protein (10133-B1)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P33681

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity

View all B7-1/CD80 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human B7-1/CD80 protein

Human B7-1/CD80 (Val35-Asn242) Accession # P33681	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val35

Predicted Molecular Mass

50.5 kDa

SDS-PAGE

66-80 kDa, under reducing conditions

Activity

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909.
The ED₅₀ for this effect is 0.025‑0.15 µg/mL in the presence of PHA.

Scientific Data Images for Recombinant Human B7-1/CD80 Fc Chimera Protein, CF

Recombinant Human B7-1/CD80 Fc Chimera Protein Bioactivity

Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 10133-B1) induces IL-2 secretion by Jurkat human acute T cell leukemia cells in the presence of PHA. The ED₅₀ for this effect is 0.025-0.15 μg/mL.

Recombinant Human B7-1/CD80 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 10133-B1) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 66-80 kDa and 130-160 kDa, respectively.

Binding of Human B7-1/CD80 to CTLA-4 Fc Avi-tag Protein by surface plasmon resonance (SPR).

Avi-tag Biotinylated Recombinant Human CTLA-4 Fc protein (AVI7268) was immobilized on a Biacore Sensor Chip CM5 via the Avi-tag biotin, and binding to Recombinant Human B7-1/CD80 Fc protein (Catalog # 10133-B1) was measured at a concentration range between 82 pM and 21 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.2511 nM.

Formulation, Preparation, and Storage

10133-B1

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: B7-1/CD80

B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses (1). Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response (2-6). Mature human B7-1 consists of a 208 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain (7). Within the ECD, human B7-1 shares 50% aa sequence identity with mouse B7-1. Alternative splicing generates a 30 kDa soluble isoform that lacks the transmembrane segment and retains the ability to bind CD28 and CTLA-4 and an isoform that lacks the second Ig-like domain and the transmembrane segment (8). Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4 (1). B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells (2).